1] Institute for Immunology, University Medical Center of Mainz, Langenbeckstrasse 1, 55101 Mainz, Germany [2].
Nat Nanotechnol. 2013 Oct;8(10):772-81. doi: 10.1038/nnano.2013.181. Epub 2013 Sep 22.
In biological fluids, proteins bind to the surface of nanoparticles to form a coating known as the protein corona, which can critically affect the interaction of the nanoparticles with living systems. As physiological systems are highly dynamic, it is important to obtain a time-resolved knowledge of protein-corona formation, development and biological relevancy. Here we show that label-free snapshot proteomics can be used to obtain quantitative time-resolved profiles of human plasma coronas formed on silica and polystyrene nanoparticles of various size and surface functionalization. Complex time- and nanoparticle-specific coronas, which comprise almost 300 different proteins, were found to form rapidly (<0.5 minutes) and, over time, to change significantly in terms of the amount of bound protein, but not in composition. Rapid corona formation is found to affect haemolysis, thrombocyte activation, nanoparticle uptake and endothelial cell death at an early exposure time.
在生物流体中，蛋白质会与纳米颗粒表面结合，形成一层被称为“蛋白质冠”的涂层，这可能会极大地影响纳米颗粒与生命系统的相互作用。由于生理系统具有高度动态性，因此获得关于蛋白质冠形成、发展和生物学相关性的时间分辨知识非常重要。在这里，我们展示了无标记快照蛋白质组学可用于获得在不同大小和表面功能化的二氧化硅和聚苯乙烯纳米颗粒上形成的人血浆冠的定量时间分辨谱。发现复杂的、随时间变化的、具有纳米颗粒特异性的蛋白质冠能够快速（<0.5 分钟）形成，并且随着时间的推移，结合蛋白质的数量会发生显著变化，但组成不变。快速的蛋白质冠形成会在早期暴露时间内影响溶血、血小板激活、纳米颗粒摄取和内皮细胞死亡。
