Nguyen Thuy-Duong Thi, Moon So-Hee, Oh Tae-Ju, Park Il-Song, Lee Min-Ho, Bae Tae-Sung
Department of Dental Biomaterials and Institute of Oral Bioscience, Brain Korea 21 Plus Project, School of Dentistry, Chonbuk National University, Jeonju, 561-756, South Korea.
J Biomed Mater Res B Appl Biomater. 2015 Apr;103(3):641-8. doi: 10.1002/jbm.b.33210. Epub 2014 Jun 28.
This study investigated the effects of anodization-cyclic precalcification-heat (APH) treatment on the bonding ability of Ca-P coating to the parent metal and osseointegration of Ti-6Al-7Nb implants. Eighteen Ti-6Al-7Nb discs, 9 untreated and 9 APH-treated, were cultured with osteoblast cells in vitro, and the cellular differentiation ability was assayed at 1, 2, and 3 weeks. For in vivo testing, 28 Ti-6Al-7Nb implants (14 implants of each group) were inserted to rat tibias, and after each 4 and 6 weeks of implantation, bone bonding, and osseointegration were evaluated through removal torque and histological analysis. Osteoblast-culturing showed twice as much of the alkaline phosphatase activity on the treated surface at 3 weeks than on the untreated surface (p < 0.05). The treated implants exhibited higher removal torque values than the untreated ones (15.5 vs. 1.8 Ncm at 4 weeks and 19.7 vs. 2.6 Ncm at 6 weeks, p < 0.05). Moreover, the excellent bonding quality of coats was confirmed by the existence of cohesive fractures on the surface of removed APH implants (field emission scanning electron microscopy and histological observation). Within the limits of this study, it can be concluded that the APH treatment significantly enhanced osseointegration of the Ti-6Al-7Nb implant, with the stable bonding between the coating and the implant surface.
本研究调查了阳极氧化-循环预钙化-加热（APH）处理对钙磷涂层与母材金属结合能力以及Ti-6Al-7Nb种植体骨整合的影响。18个Ti-6Al-7Nb圆盘，9个未处理，9个经APH处理，在体外与成骨细胞共培养，并在1、2和3周时测定细胞分化能力。对于体内测试，将28个Ti-6Al-7Nb种植体（每组14个种植体）植入大鼠胫骨，在植入后每4周和6周，通过去除扭矩和组织学分析评估骨结合和骨整合情况。成骨细胞培养显示，在3周时，处理过的表面上碱性磷酸酶活性是未处理表面的两倍（p < 0.05）。处理过的种植体的去除扭矩值高于未处理的种植体（4周时为15.5对1.8 Ncm，6周时为19.7对2.6 Ncm，p < 0.05）。此外，通过去除的APH种植体表面存在内聚性骨折（场发射扫描电子显微镜和组织学观察）证实了涂层具有优异的结合质量。在本研究的范围内，可以得出结论，APH处理显著增强了Ti-6Al-7Nb种植体的骨整合，涂层与种植体表面之间具有稳定的结合。
