Reeve Wayne, Drew Elizabeth, Ballard Ross, Melino Vanessa, Tian Rui, De Meyer Sofie, Brau Lambert, Ninawi Mohamed, Daligault Hajnalka, Davenport Karen, Erkkila Tracy, Goodwin Lynne, Gu Wei, Munk Christine, Teshima Hazuki, Xu Yan, Chain Patrick, Kyrpides Nikos
Centre for Rhizobium Studies, Murdoch University, Western Australia, Australia.
South Australian Research and Development Institute, Urrbrae, South Australia, Australia.
Stand Genomic Sci. 2013 Dec 15;9(2):232-42. doi: 10.4056/sigs.4478252. eCollection 2013 Dec 20.
Rhizobium leguminosarum bv. trifolii SRDI943 (strain syn. V2-2) is an aerobic, motile, Gram-negative, non-spore-forming rod that was isolated from a root nodule of Trifolium michelianum Savi cv. Paradana that had been grown in soil collected from a mixed pasture in Victoria, Australia. This isolate was found to have a broad clover host range but was sub-optimal for nitrogen fixation with T. subterraneum (fixing 20-54% of reference inoculant strain WSM1325) and was found to be totally ineffective with the clover species T. polymorphum and T. pratense. Here we describe the features of R. leguminosarum bv. trifolii strain SRDI943, together with genome sequence information and annotation. The 7,412,387 bp high-quality-draft genome is arranged into 5 scaffolds of 5 contigs, contains 7,317 protein-coding genes and 89 RNA-only encoding genes, and is one of 100 rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project.
三叶草根瘤菌三叶草生物变种SRDI943（菌株同义名V2-2）是一种需氧、具运动性、革兰氏阴性、不形成芽孢的杆菌，它是从生长在澳大利亚维多利亚州一块混合牧场采集土壤中的米氏三叶草品种Paradana的根瘤中分离得到的。该分离株被发现具有广泛的三叶草宿主范围，但与地下三叶草进行固氮时效果欠佳（固氮量为参考接种菌株WSM1325的20%-54%），并且发现它对多形三叶草和草地三叶草这两种三叶草完全无效。在此，我们描述了三叶草根瘤菌三叶草生物变种SRDI943的特征，以及基因组序列信息和注释。这个7,412,387 bp的高质量草图基因组被排列成由5个重叠群组成的5个支架，包含7,317个蛋白质编码基因和89个仅编码RNA的基因，它是作为美国能源部联合基因组研究所2010年细菌和古菌基因组百科全书-根瘤菌（GEBA-RNB）项目的一部分而测序的100个根瘤菌基因组之一。
