Mahmoudvand Hossein, Ayatollahi Mousavi Seyyed Amin, Sepahvand Asghar, Sharififar Fariba, Ezatpour Behrouz, Gorohi Fatemeh, Saedi Dezaki Ebrahim, Jahanbakhsh Sareh
Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman 76169-14119, Iran ; Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad 68138-33946, Iran.
Department of Medical Parasitology and Mycology, Kerman University of Medical Sciences, Kerman 76169-14114, Iran.
ISRN Pharmacol. 2014 Mar 10;2014:602436. doi: 10.1155/2014/602436. eCollection 2014.
In this study, in vitro antidermatophytic activity against Trichophyton mentagrophytes, Trichophyton rubrum, Microsporum canis, and Microsporum gypseum was studied by disk diffusion test and assessment of minimum inhibitory concentration (MIC) using CLSI broth macrodilution method (M38-A2). Moreover, antileishmanial and cytotoxicity activity of B. vulgaris and berberine against promastigotes of Leishmania major and Leishmania tropica were evaluated by colorimetric MTT assay. The findings indicated that the various extracts of B. vulgaris particularly berberine showed high potential antidermatophytic against pathogenic dermatophytes tested with MIC values varying from 0.125 to >4 mg/mL. The results revealed that B. vulgaris extracts as well as berberine were effective in inhibiting L. major and L. tropica promastigotes growth in a dose-dependent manner with IC50 (50% inhibitory concentration) values varying from 2.1 to 26.6 μ g/mL. Moreover, it could be observed that berberine as compared with B. vulgaris exhibited more cytotoxicity against murine macrophages with CC50 (cytotoxicity concentration for 50% of cells) values varying from 27.3 to 362.6 μ g/mL. Results of this investigation were the first step in the search for new antidermatophytic and antileishmanial drugs. However, further works are required to evaluate exact effect of these extracts in animal models as well as volunteer human subjects.
在本研究中，通过纸片扩散试验以及使用CLSI肉汤大稀释法（M38 - A2）评估最低抑菌浓度（MIC），研究了对须癣毛癣菌、红色毛癣菌、犬小孢子菌和石膏样小孢子菌的体外抗皮肤癣菌活性。此外，通过比色MTT法评估了普通大黄和黄连素对硕大利什曼原虫和热带利什曼原虫前鞭毛体的抗利什曼原虫活性和细胞毒性活性。研究结果表明，普通大黄的各种提取物尤其是黄连素对所测试的致病性皮肤癣菌显示出高潜在抗皮肤癣菌活性，MIC值在0.125至>4mg/mL之间变化。结果显示，普通大黄提取物以及黄连素均能有效抑制硕大利什曼原虫和热带利什曼原虫前鞭毛体的生长，呈剂量依赖性，IC50（50%抑制浓度）值在2.1至26.6μg/mL之间变化。此外，可以观察到，与普通大黄相比，黄连素对小鼠巨噬细胞表现出更高的细胞毒性，CC50（50%细胞的细胞毒性浓度）值在27.3至362.6μg/mL之间变化。本研究结果是寻找新型抗皮肤癣菌和抗利什曼原虫药物的第一步。然而，还需要进一步的研究来评估这些提取物在动物模型以及志愿者人体受试者中的确切效果。
