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本文引用的文献

1
A novel alkyne cholesterol to trace cellular cholesterol metabolism and localization.一种新型炔烃胆固醇追踪细胞胆固醇代谢和定位。
J Lipid Res. 2014 Mar;55(3):583-91. doi: 10.1194/jlr.D044727. Epub 2013 Dec 12.
2
Bacterial lipids: metabolism and membrane homeostasis.细菌脂质：代谢与膜平衡。
Prog Lipid Res. 2013 Jul;52(3):249-76. doi: 10.1016/j.plipres.2013.02.002. Epub 2013 Mar 14.
3
Neutral loss fragmentation pattern based screening for arginine-rich natural products in Xenorhabdus and Photorhabdus.基于中性丢失碎片模式筛选 Xenorhabdus 和 Photorhabdus 中的精氨酸丰富天然产物。
Anal Chem. 2012 Aug 21;84(16):6948-55. doi: 10.1021/ac300372p. Epub 2012 Aug 6.
4
Preventing thiol-yne addition improves the specificity of strain-promoted azide-alkyne cycloaddition.预防硫醇-炔加成反应可提高应变促进的叠氮化物-炔环加成反应的特异性。
Bioconjug Chem. 2012 Mar 21;23(3):392-8. doi: 10.1021/bc200365k. Epub 2012 Mar 8.
5
A natural prodrug activation mechanism in nonribosomal peptide synthesis.非核糖体肽合成中的天然前药激活机制。
Nat Chem Biol. 2011 Sep 18;7(12):888-90. doi: 10.1038/nchembio.688.
6
Bioorthogonal chemical reporters for analyzing protein lipidation and lipid trafficking.用于分析蛋白质脂化和脂质转运的生物正交化学报告物。
Acc Chem Res. 2011 Sep 20;44(9):699-708. doi: 10.1021/ar200063v. Epub 2011 Jun 15.
7
Cu-free click cycloaddition reactions in chemical biology.无铜点击环加成反应在化学生物学中的应用。
Chem Soc Rev. 2010 Apr;39(4):1272-9. doi: 10.1039/b901970g.
8
A hydrophilic azacyclooctyne for Cu-free click chemistry.一种用于无铜点击化学的亲水性氮杂环辛炔。
Org Lett. 2008 Jul 17;10(14):3097-9. doi: 10.1021/ol801141k. Epub 2008 Jun 13.
9
Identification and functional expression of a Delta9-fatty acid desaturase from Psychrobacter urativorans in Escherichia coli.嗜尿冷动菌Delta9-脂肪酸去饱和酶在大肠杆菌中的鉴定及功能表达
Lipids. 2008 Mar;43(3):207-13. doi: 10.1007/s11745-007-3150-5. Epub 2008 Feb 7.
10
Control of fatty acid desaturation: a mechanism conserved from bacteria to humans.脂肪酸去饱和作用的调控：一种从细菌到人类都保守的机制。
Mol Microbiol. 2006 Dec;62(6):1507-14. doi: 10.1111/j.1365-2958.2006.05484.x.

ω-叠氮脂肪酸作为检测脂肪酸生物合成、降解和修饰的探针。

ω-Azido fatty acids as probes to detect fatty acid biosynthesis, degradation, and modification.

作者信息

Pérez Alexander J, Bode Helge B

机构信息

Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Goethe Universität Frankfurt, 60438 Frankfurt am Main, Germany.

出版信息

J Lipid Res. 2014 Sep;55(9):1897-901. doi: 10.1194/jlr.M047969. Epub 2014 Jul 10.

DOI:10.1194/jlr.M047969
PMID:25013232
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4617356/
Abstract

FAs play a central role in the metabolism of almost all known cellular life forms. Although GC-MS is regarded as a standard method for FA analysis, other methods, such as HPLC/MS, are nowadays widespread but are rarely applied to FA analysis. Here we present azido-FAs as probes that can be used to study FA biosynthesis (elongation, desaturation) or degradation (β-oxidation) upon their uptake, activation, and metabolic conversion. These azido-FAs are readily accessible by chemical synthesis and their metabolic products can be easily detected after click-chemistry based derivatization with high sensitivity by HPLC/MS, contributing a powerful tool to FA analysis, and hence, lipid analysis in general.

摘要

脂肪酸在几乎所有已知细胞生命形式的代谢中都起着核心作用。尽管气相色谱-质谱联用（GC-MS）被视为脂肪酸分析的标准方法，但如今其他方法，如高效液相色谱/质谱联用（HPLC/MS）已广泛应用，不过很少用于脂肪酸分析。在此，我们展示了叠氮脂肪酸作为探针，在其摄取、活化和代谢转化后，可用于研究脂肪酸生物合成（延长、去饱和）或降解（β-氧化）。这些叠氮脂肪酸可通过化学合成轻易获得，并且在用基于点击化学的衍生化试剂进行衍生化后，其代谢产物能够通过高效液相色谱/质谱联用（HPLC/MS）以高灵敏度轻松检测到，这为脂肪酸分析乃至一般脂质分析提供了一个强大的工具。