ω-叠氮脂肪酸作为检测脂肪酸生物合成、降解和修饰的探针。
ω-Azido fatty acids as probes to detect fatty acid biosynthesis, degradation, and modification.
作者信息
Pérez Alexander J, Bode Helge B
机构信息
Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Goethe Universität Frankfurt, 60438 Frankfurt am Main, Germany.
出版信息
J Lipid Res. 2014 Sep;55(9):1897-901. doi: 10.1194/jlr.M047969. Epub 2014 Jul 10.
Abstract
FAs play a central role in the metabolism of almost all known cellular life forms. Although GC-MS is regarded as a standard method for FA analysis, other methods, such as HPLC/MS, are nowadays widespread but are rarely applied to FA analysis. Here we present azido-FAs as probes that can be used to study FA biosynthesis (elongation, desaturation) or degradation (β-oxidation) upon their uptake, activation, and metabolic conversion. These azido-FAs are readily accessible by chemical synthesis and their metabolic products can be easily detected after click-chemistry based derivatization with high sensitivity by HPLC/MS, contributing a powerful tool to FA analysis, and hence, lipid analysis in general.
摘要
脂肪酸在几乎所有已知细胞生命形式的代谢中都起着核心作用。尽管气相色谱-质谱联用（GC-MS）被视为脂肪酸分析的标准方法，但如今其他方法，如高效液相色谱/质谱联用（HPLC/MS）已广泛应用，不过很少用于脂肪酸分析。在此，我们展示了叠氮脂肪酸作为探针，在其摄取、活化和代谢转化后，可用于研究脂肪酸生物合成（延长、去饱和）或降解（β-氧化）。这些叠氮脂肪酸可通过化学合成轻易获得，并且在用基于点击化学的衍生化试剂进行衍生化后，其代谢产物能够通过高效液相色谱/质谱联用（HPLC/MS）以高灵敏度轻松检测到，这为脂肪酸分析乃至一般脂质分析提供了一个强大的工具。
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