Natural Health Products and Metabolic Diseases Laboratory, Department of Pharmacology, University of Montreal, Station Centre-Ville, P.O. Box 6128, Montreal, QC, Canada H3C 3J7 ; Phytochemistry, Medicinal Plant and Ethnopharmacology Laboratory, Department of Biology, University of Ottawa, Ottawa, ON, Canada K1N 6N5 ; Canadian Institutes of Health Research Team in Aboriginal Antidiabetic Medicines and Montreal Diabetes Research Center, Canada ; Department of Pharmacognosy, University of Beni Suef, Beni Suef 62511, Egypt.
Natural Health Products and Metabolic Diseases Laboratory, Department of Pharmacology, University of Montreal, Station Centre-Ville, P.O. Box 6128, Montreal, QC, Canada H3C 3J7 ; Phytochemistry, Medicinal Plant and Ethnopharmacology Laboratory, Department of Biology, University of Ottawa, Ottawa, ON, Canada K1N 6N5 ; Canadian Institutes of Health Research Team in Aboriginal Antidiabetic Medicines and Montreal Diabetes Research Center, Canada.
Evid Based Complement Alternat Med. 2014;2014:645812. doi: 10.1155/2014/645812. Epub 2014 Jun 10.
Vaccinium vitis-idaea, commonly known as lingonberry, has been identified among species used by the Cree of Eeyou Istchee of northern Quebec to treat symptoms of diabetes. In a previous study, the ethanol extract of berries of V. vitis-idaea enhanced glucose uptake in C2C12 muscle cells via stimulation of AMP-activated protein kinase (AMPK) pathway. The purpose of this study was to examine the effect of plant extract in a dietary mouse model of mild type 2 diabetes. C57BL/6 mice fed a high-fat diet (HFD, ∼35% lipids) for 8 weeks that become obese and insulin-resistant (diet-induced obesity, DIO) were used. Treatment began by adding V. vitis-idaea extract to HFD at 3 different concentrations (125, 250, and 500 mg/Kg) for a subsequent period of 8 weeks (total HFD, 16 weeks). The plant extract significantly decreased glycemia and strongly tended to decrease insulin levels in this model. This was correlated with a significant increase in GLUT4 content and activation of the AMPK and Akt pathways in skeletal muscle. V. vitis-idaea treatment also improved hepatic steatosis by decreasing hepatic triglyceride levels and significantly activated liver AMPK and Akt pathways. The results of the present study confirm that V. vitis-idaea represents a culturally relevant treatment option for Cree diabetics and pave the way to clinical studies.
越橘，俗称蔓越莓，在魁北克北部易洛魁族用于治疗糖尿病症状的物种中被发现。在之前的一项研究中，越橘浆果的乙醇提取物通过刺激 AMP 激活的蛋白激酶（AMPK）通路增强 C2C12 肌肉细胞的葡萄糖摄取。本研究的目的是在轻度 2 型糖尿病的饮食诱导肥胖小鼠模型中检查植物提取物的作用。8 周高脂饮食（HFD，约 35%脂质）喂养的 C57BL/6 肥胖和胰岛素抵抗（饮食诱导肥胖，DIO）小鼠被用于研究。通过在 HFD 中添加 3 种不同浓度（125、250 和 500mg/Kg）的越橘提取物开始治疗，随后持续 8 周（总 HFD，16 周）。植物提取物可显著降低血糖，且明显倾向于降低该模型中的胰岛素水平。这与骨骼肌中 GLUT4 含量的显著增加以及 AMPK 和 Akt 途径的激活相关。越橘提取物还通过降低肝甘油三酯水平显著激活肝脏 AMPK 和 Akt 途径，改善肝脏脂肪变性。本研究的结果证实，越橘提取物是易洛魁族糖尿病患者具有文化相关性的治疗选择，并为临床研究铺平了道路。
