Peng Hanchuan, Tang Jianyong, Xiao Hang, Bria Alessandro, Zhou Jianlong, Butler Victoria, Zhou Zhi, Gonzalez-Bellido Paloma T, Oh Seung W, Chen Jichao, Mitra Ananya, Tsien Richard W, Zeng Hongkui, Ascoli Giorgio A, Iannello Giulio, Hawrylycz Michael, Myers Eugene, Long Fuhui
1] Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA [2] Allen Institute for Brain Science, 551 North 34th Street, Suite 200, Seattle, Washington 98103, USA.
1] Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA [2].
Nat Commun. 2014 Jul 11;5:4342. doi: 10.1038/ncomms5342.
Three-dimensional (3D) bioimaging, visualization and data analysis are in strong need of powerful 3D exploration techniques. We develop virtual finger (VF) to generate 3D curves, points and regions-of-interest in the 3D space of a volumetric image with a single finger operation, such as a computer mouse stroke, or click or zoom from the 2D-projection plane of an image as visualized with a computer. VF provides efficient methods for acquisition, visualization and analysis of 3D images for roundworm, fruitfly, dragonfly, mouse, rat and human. Specifically, VF enables instant 3D optical zoom-in imaging, 3D free-form optical microsurgery, and 3D visualization and annotation of terabytes of whole-brain image volumes. VF also leads to orders of magnitude better efficiency of automated 3D reconstruction of neurons and similar biostructures over our previous systems. We use VF to generate from images of 1,107 Drosophila GAL4 lines a projectome of a Drosophila brain.
三维（3D）生物成像、可视化和数据分析迫切需要强大的3D探索技术。我们开发了虚拟手指（VF），通过单指操作（如计算机鼠标的笔划、点击或从计算机可视化的图像二维投影平面进行缩放）在体图像的3D空间中生成3D曲线、点和感兴趣区域。VF为蛔虫、果蝇、蜻蜓、小鼠、大鼠和人类的3D图像采集、可视化和分析提供了高效方法。具体而言，VF能够实现即时3D光学放大成像、3D自由形式光学显微手术以及对数TB全脑图像体积进行3D可视化和注释。与我们之前的系统相比，VF还使神经元和类似生物结构的自动3D重建效率提高了几个数量级。我们使用VF从1107个果蝇GAL4品系的图像中生成果蝇大脑的投射图谱。
