Nadal D, Wunderli W, Briner H, Hansen K
Department of Pediatrics, University of Zurich, Switzerland.
Eur J Clin Microbiol Infect Dis. 1989 Nov;8(11):992-5. doi: 10.1007/BF01967572.
Sera from 259 forestry workers and 100 blood donors in the Canton of Solothurn, Switzerland, were tested for IgG antibodies to Borrelia burgdorferi in two EIAs using as antigen either sonic extract of whole organisms or purified flagella. Applying a 95% specific cut-off value based on results in the sera of 100 blood donors, 86 (33%) and 91 (35%) of the forestry workers respectively showed an elevated specific IgG level in the two EIAs. None of the 259 forestry workers had clinical signs of active infection at the time blood was taken, and only nine could recall experiencing erythema-migrans-like skin lesions within the last ten years. Thus, asymptomatic infections must be frequent. Elevated specific antibody levels increased significantly with the age of the forestry workers (p less than 0.0001) and the duration of occupational exposure to ticks (p = 0.0001). Thus serological results in individuals with high exposure to ticks must be interpreted with caution in view of the high a priori prevalence of antibodies to Borrelia burgdorferi in such persons. The prevalence of antibody to the Borrelia burgdorferi flagellum in a control population not selected for tick exposure, in this case blood donors, seems to be independent of geographical origin.
在瑞士索洛图恩州，对259名林业工人和100名献血者的血清进行了检测，采用两种酶免疫测定法检测抗伯氏疏螺旋体的IgG抗体，抗原分别为全菌超声提取物或纯化鞭毛。根据100名献血者血清的检测结果设定95%的特异性临界值，在这两种酶免疫测定法中，分别有86名（33%）和91名（35%）林业工人的特异性IgG水平升高。在采集血液时，259名林业工人均无活动性感染的临床症状，只有9人回忆起在过去十年内曾出现过游走性红斑样皮肤损害。因此，无症状感染想必很常见。特异性抗体水平升高与林业工人的年龄（p<0.0001）以及职业性接触蜱的时长（p = 0.0001）显著相关。因此，鉴于蜱高暴露人群中抗伯氏疏螺旋体抗体的先验患病率很高，对这类个体的血清学检测结果必须谨慎解读。在未因蜱暴露而选择的对照人群（在此为献血者）中，抗伯氏疏螺旋体鞭毛抗体的患病率似乎与地理来源无关。
