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不同种类蔬菜和沙拉中幽门螺杆菌的检测

Detection of Helicobacter pylori in Various Types of Vegetables and Salads.

作者信息

Atapoor Shahrzad, Safarpoor Dehkordi Farhad, Rahimi Ebrahim

机构信息

Faculty of Agriculture and Natural Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, IR Iran.

Young Researchers and Elites Club, Shahrekord Branch, Islamic Azad University, Shahrekord, IR Iran.

出版信息

Jundishapur J Microbiol. 2014 May;7(5):e10013. doi: 10.5812/jjm.10013. Epub 2014 May 1.

DOI:10.5812/jjm.10013
PMID:25147709
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4138632/
Abstract

BACKGROUND

There is a possibility for the presence of Helicobacter pylori in vegetables due to their close contact with polluted water, soil and feces.

OBJECTIVES

This study was carried out to detect the presence of H. pylori in vegetables and salads in Iran.

MATERIALS AND METHODS

In total, 460 vegetable and salad samples were collected and transferred immediately to the laboratory. All samples were cultured and tested for the presence of H. pylori using the Polymerase Chain Reaction technique.

RESULTS

The results showed that 44 of 460 samples (9.56%) were positive for H. pylori using the culture method. The Polymerase Chain Reaction technique showed that 50 of 460 samples (10.86%) were positive for H. pylori. Un-washed leek, traditional salad, un-washed basil and un-washed lettuce were the most commonly contaminated samples. The presence of the bacteria in various vegetables was statistically significant (P < 0.05).

CONCLUSIONS

Vegetables are a new source of H. pylori and accurate washing of vegetables improves such contaminations.

摘要

背景

蔬菜因与受污染的水、土壤和粪便密切接触,存在感染幽门螺杆菌的可能性。

目的

本研究旨在检测伊朗蔬菜和沙拉中幽门螺杆菌的存在情况。

材料与方法

共收集460份蔬菜和沙拉样本,并立即送往实验室。所有样本均进行培养,并使用聚合酶链反应技术检测幽门螺杆菌的存在情况。

结果

结果显示,采用培养法时,460份样本中有44份(9.56%)幽门螺杆菌检测呈阳性。聚合酶链反应技术显示,460份样本中有50份(10.86%)幽门螺杆菌检测呈阳性。未清洗的韭菜、传统沙拉、未清洗的罗勒和未清洗的生菜是最常受污染的样本。各种蔬菜中细菌的存在具有统计学意义(P < 0.05)。

结论

蔬菜是幽门螺螺螺螺杆菌的新来源,对蔬菜进行正确清洗可减少此类污染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebcf/4138632/58b57e33c00d/jjm-07-10013-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebcf/4138632/58b57e33c00d/jjm-07-10013-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ebcf/4138632/58b57e33c00d/jjm-07-10013-g001.jpg

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