Department of Nursing, Hungkuang University, Shalu County, Taichung Hsien, Taiwan.
Pharm Biol. 2013 Jan;51(1):84-95. doi: 10.3109/13880209.2012.711840. Epub 2012 Oct 8.
The extraction method and the crude wound healing effects of sacchachitin from Ganoderma tsugae Murr. (Ganodermataceae) has been cited. However, its purity is still largely limited.
An improvement of the fractionation protocol to purify the sacchachitin from Ganoderma lucidum L. (Ganodermataceae) (SGL) is needed.
Fruiting bodies were extracted with double distilled water and subsequently the residue treated with 95% ethanol and then 40% ethanol. After being filtered, the pH of the supernatant was adjusted to 4.0 with 1 N HCl and lyophilized. The supernatant was added (3:1 v/v) ethanol, the precipitate was collected, 2% NaOH was added and refluxed. The supernatant was collected with pH adjusted to 4.0, then treated with 10% potassium hydroxide (KOH) with repeating acid precipitation and (3:1) ethanol precipitation twice more to obtain the sacchachitin.
SGL had a hexosamine content 16.3% (w/w), firmly linked to a talomannan. Its Fourier Transform Infrared Spectroscopy (FTIR) spectrum revealed specific absorption (in cm(-1)) ν(O-H) 3455.5 b,s, amide ν(C=O) 1678.5, and amide I° δ(N-H) 1550.4. The percentage deacetylation degree was 37.6 and 39.4% for SGL and MSC, respectively. As contrast, MSC contained only 6.6% of hexosamine with a low protein/carbohydrate ratio 0.35 comparing to 0.82 for SGL. SGL was only moderately strong antioxidant regarding the anti-DPPH, antihydroxyl free radical, and antisuperoxide anion capabilities, exhibiting an IC(33) values of 10 mg/mL (the highest scavenging capability never exceeding 33%), 0.9 mg/mL, and 4.8 mg/mL, respectively.
We have successfully isolated the pure sacchachitin from the fruiting bodies of G. lucidum that exhibits potent antioxidative activity and may be useful in fabrication of the artificial skin composite substitute.
已引用了从密纹薄芝（多孔菌科）中提取几丁质和粗伤口愈合效果的提取方法。然而，其纯度在很大程度上仍然受到限制。
需要改进从灵芝（多孔菌科）（SGL）中分离几丁质的分馏方案。
用双蒸水提取子实体，然后用 95%乙醇和 40%乙醇处理残渣。过滤后，用 1N HCl 将上清液的 pH 值调至 4.0 并冻干。在上清液中加入（3:1v/v）乙醇，收集沉淀物，加入 2%NaOH 并回流。收集 pH 值调至 4.0 的上清液，然后用 10%氢氧化钾（KOH）处理，重复酸沉淀两次，再用（3:1）乙醇沉淀两次，得到几丁质。
SGL 的己糖胺含量为 16.3%（w/w），与塔洛甘露聚糖牢固结合。其傅里叶变换红外光谱（FTIR）谱显示出特定的吸收（cm(-1)）ν（O-H）3455.5b，s，酰胺ν（C=O）1678.5 和酰胺 I°δ（N-H）1550.4。脱乙酰度分别为 37.6%和 39.4%，SGL 和 MSC。相比之下，MSC 仅含有 6.6%的己糖胺，其蛋白质/碳水化合物比为 0.35，而 SGL 为 0.82。SGL 仅具有中等强度的抗氧化能力，对 DPPH、羟自由基和超氧阴离子的清除能力，其 IC（33）值分别为 10mg/mL（最高清除能力从未超过 33%）、0.9mg/mL 和 4.8mg/mL。
我们已经成功地从灵芝子实体中分离出纯几丁质，它具有很强的抗氧化活性，可用于制造人工皮肤复合替代物。
