López-Roig Marc, Bourhy Hervé, Lavenir Rachel, Serra-Cobo Jordi
Departament de Biologia Animal, Facultat de Biologia, Universitat de Barcelona, Barcelona, 08028, Spain.
Institut Pasteur, Unité Dynamique des Lyssavirus et Adaptation à l'Hôte, WHO Collaborating Centre for Reference and Research on Rabies, Paris, 75724, France.
Viruses. 2014 Sep 4;6(9):3386-99. doi: 10.3390/v6093386.
We report an active surveillance study of the occurrence of specific antibodies to European Bat Lyssavirus Type 1 (EBLV-1) in bat species, scarcely studied hitherto, that share the same refuge. From 2004 to 2012, 406 sera were obtained from nine bat species. Blood samples were subjected to a modified fluorescent antibody virus neutralization test to determine the antibody titer. EBLV-1-neutralizing antibodies were detected in six of the nine species analyzed (Pipistrellus pipistrellus, P. kuhlii, Hypsugo savii, Plecotus austriacus, Eptesicus serotinus and Tadarida teniotis). Among all bats sampled, female seroprevalence (20.21%, 95% CI: 14.78%-26.57%) was not significantly higher than the seroprevalence in males (15.02%, 95% CI: 10.51%-20.54%). The results showed that the inter-annual variation in the number of seropositive bats in T. teniotis and P. austriacus showed a peak in 2007 (>70% of EBLV-1 prevalence). However, significant differences were observed in the temporal patterns of the seroprevalence modeling of T. teniotis and P. austriacus. The behavioral ecology of these species involved could explain the different annual fluctuations in EBLV-1 seroprevalence.
我们报告了一项主动监测研究，该研究针对迄今鲜有研究的、共享同一栖息地的蝙蝠物种中欧洲1型蝙蝠狂犬病病毒（EBLV-1）特异性抗体的出现情况展开。在2004年至2012年期间，从9种蝙蝠中采集了406份血清样本。对血液样本进行改良荧光抗体病毒中和试验以确定抗体滴度。在所分析的9种蝙蝠中，有6种检测到了EBLV-1中和抗体（伏翼、库氏伏翼、萨氏长翼蝠、奥氏鼠耳蝠、血清蝙蝠和埃及墓蝠）。在所有采样蝙蝠中，雌性的血清阳性率（20.21%，95%置信区间：14.78%-26.57%）并不显著高于雄性的血清阳性率（15.02%，95%置信区间：10.51%-20.54%）。结果显示，埃及墓蝠和奥氏鼠耳蝠中血清阳性蝙蝠数量的年际变化在2007年出现峰值（EBLV-1流行率>70%）。然而，埃及墓蝠和奥氏鼠耳蝠血清阳性率建模的时间模式存在显著差异。所涉及的这些物种的行为生态学可以解释EBLV-1血清阳性率的不同年度波动情况。
