Brookes Sharon M, Aegerter James N, Smith Graham C, Healy Derek M, Jolliffe Tracey A, Swift Susan M, Mackie Iain J, Pritchard J Stewart, Racey Paul A, Moore Niall P, Fooks Anthony R
World Health Organization Collaborating Centre for the Characterisation of Rabies and Rabies-Related Viruses, Surrey, United Kingdom.
Emerg Infect Dis. 2005 Apr;11(4):572-8. doi: 10.3201/eid1104.040920.
We report the first seroprevalence study of the occurrence of specific antibodies to European bat lyssavirus type 2 (EBLV-2) in Daubenton's bats. Bats were captured from 19 sites across eastern and southern Scotland. Samples from 198 Daubenton's bats, 20 Natterer's bats, and 6 Pipistrelle's bats were tested for EBLV-2. Blood samples (N = 94) were subjected to a modified fluorescent antibody virus neutralization test to determine antibody titer. From 0.05% to 3.8% (95% confidence interval) of Daubenton's bats were seropositive. Antibodies to EBLV-2 were not detected in the 2 other species tested. Mouth swabs (N = 218) were obtained, and RNA was extracted for a reverse transcription-polymerase chain reaction (RT-PCR). The RT-PCR included pan lyssavirus-primers (N gene) and internal PCR control primers for ribosomal RNA. EBLV-2 RNA was not detected in any of the saliva samples tested, and live virus was not detected in virus isolation tests.
我们报告了对道氏鼠耳蝠体内欧洲蝙蝠狂犬病毒2型（EBLV - 2）特异性抗体出现情况的首次血清流行率研究。蝙蝠从苏格兰东部和南部的19个地点捕获。对198只道氏鼠耳蝠、20只纳氏鼠耳蝠和6只伏翼蝠的样本进行了EBLV - 2检测。对血液样本（N = 94）进行改良荧光抗体病毒中和试验以确定抗体滴度。0.05%至3.8%（95%置信区间）的道氏鼠耳蝠血清呈阳性。在另外两种受试物种中未检测到EBLV - 2抗体。获取了口腔拭子（N = 218），并提取RNA用于逆转录聚合酶链反应（RT - PCR）。RT - PCR包括通用狂犬病毒引物（N基因）和核糖体RNA的内部PCR对照引物。在任何受试唾液样本中均未检测到EBLV - 2 RNA，并且在病毒分离试验中未检测到活病毒。
