Christianson Sara, Voth Dylan, Wolfe Joyce, Sharma Meenu K
National Reference Centre for Mycobacteriology, National Microbiology Laboratory Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada.
PLoS One. 2014 Sep 26;9(9):e108911. doi: 10.1371/journal.pone.0108911. eCollection 2014.
The critical concentration (CC) for ethambutol testing on the Bactec MGIT 960 M. tuberculosis susceptibility testing has been questioned in recent publications. In this study, we correlate susceptibility results from the Bactec 460, MGIT 960 and embB gene sequencing to determine if the Bactec MGIT 960 adequately detects ethambutol resistance. We discovered discrepancies between the methods that highlight a need to re-evaluate ethambutol susceptibility testing recommendations, namely by considering lowering currently recommended CC on the MGIT 960. Further studies on the clinical significance of low-level ethambutol resistance are also required.
近期的一些出版物对在Bactec MGIT 960系统上进行结核分枝杆菌乙胺丁醇药敏试验的临界浓度（CC）提出了质疑。在本研究中，我们将Bactec 460、MGIT 960的药敏结果与embB基因测序结果进行关联，以确定Bactec MGIT 960能否充分检测出乙胺丁醇耐药性。我们发现不同方法之间存在差异，这凸显了重新评估乙胺丁醇药敏试验建议的必要性，即考虑降低目前MGIT 960上推荐的临界浓度。还需要进一步研究低水平乙胺丁醇耐药性的临床意义。
