Department of Biological and Chemical Engineering, Chongqing University of Education, China.
College of Food Science, Southwest University, 2 Tiansheng Road, Beibei District, Chongqing 400067, China.
Nutr Res Pract. 2014 Oct;8(5):487-93. doi: 10.4162/nrp.2014.8.5.487. Epub 2014 Aug 30.
BACKGROUND/OBJECTIVES: D. candidum is a traditional Chinese food or medicine widely used in Asia. There has been little research into the anticancer effects of D. candidum, particularly the effects in colon cancer cells. The aim of this study was to investigate the anticancer effects of D. candidum in vitro and in vivo.
MATERIALS/METHODS: The in vitro anti-cancer effects on HCT-116 colon cancer cells and in vivo anti-metastatic effects of DCME (Dendrobium canidum methanolic extract) were examined using the experimental methods of MTT assay, DAPI staining, flow cytometry analysis, RT-PCR, and Western blot analysis.
At a concentration of 1.0 mg/mL, DCME inhibited the growth of HCT-116 cells by 84%, which was higher than at concentrations of 0.5 and 0.25 mg/mL. Chromatin condensation and formation of apoptotic bodies were observed in cancer cells cultured with DCME as well. In addition, DCME induced significant apoptosis in cancer cells by upregulation of Bax, caspase 9, and caspase 3, and downregulation of Bcl-2. Expression of genes commonly associated with inflammation, NF-κB, iNOS, and COX-2, was significantly downregulated by DCME. DCME also exerted an anti-metastasis effect on cancer cells as demonstrated by decreased expression of MMP genes and increased expression of TIMPs, which was confirmed by the inhibition of induced tumor metastasis in colon 26-M3.1 cells in BALB/c mice.
Our results demonstrated that D. candidum had a potent in vitro anti-cancer effect, induced apoptosis, exhibited anti-inflammatory activities, and exerted in vivo anti-metastatic effects.
背景/目的：白参是一种在亚洲广泛使用的传统食品或药物。对白参的抗癌作用，特别是对结肠癌细胞的作用，研究甚少。本研究旨在探讨白参在体外和体内的抗癌作用。
材料/方法：采用 MTT 法、DAPI 染色法、流式细胞术分析、RT-PCR 和 Western blot 分析等实验方法，研究 DCME（铁皮石斛甲醇提取物）对 HCT-116 结肠癌细胞的体外抗癌作用和体内转移抑制作用。
浓度为 1.0mg/mL 时，DCME 抑制 HCT-116 细胞生长 84%，高于 0.5mg/mL 和 0.25mg/mL。用 DCME 培养的癌细胞可见染色质浓缩和凋亡小体形成。此外，DCME 通过上调 Bax、caspase 9 和 caspase 3，下调 Bcl-2，诱导癌细胞发生显著凋亡。与炎症相关的基因 NF-κB、iNOS 和 COX-2 的表达也被 DCME 显著下调。DCME 还通过降低 MMP 基因的表达和增加 TIMPs 的表达，对癌细胞发挥了抗转移作用，这在 BALB/c 小鼠中结肠 26-M3.1 细胞诱导的肿瘤转移抑制实验中得到了证实。
我们的研究结果表明，白参具有很强的体外抗癌作用，能诱导细胞凋亡，具有抗炎活性，并具有体内抗转移作用。
