Hao Lijing, Yang Hui, Du Chang, Fu Xiaoling, Zhao Naru, Xu Suju, Cui Fuzhai, Mao Chuanbin, Wang Yingjun
School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China ; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China.
Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, PR China.
J Mater Chem B. 2014 Aug 14;2(30):4794-4801. doi: 10.1039/C4TB00597J.
Self-assembled monolayers (SAMs) of alkanethiols on gold have been employed as model substrates to investigate the effects of surface chemistry on cell behavior. However, few studies were dedicated to the substrates with a controlled wettability in studying stem cell fate. Here, mixed hydroxyl (-OH) and methyl (-CH) terminated SAMs were prepared to form substrates with varying wettability, which were used to study the effects of wettability on the adhesion, spreading, proliferation and osteogenic differentiation of mesenchymal stem cells (MSCs) from human and mouse origins. The numbers of adhered human fetal MSCs (hMSCs) and mouse bone marrow MSCs (mMSCs) were maximized on -OH/-CH mixed SAMs with a water contact angle of 4070° and 7090°, respectively. Hydrophilic mixed SAMs with a water contact angle of 2070° also promoted the spreading of both hMSCs and mMSCs. Both hMSCs and mMSCs proliferation was most favored on hydrophilic SAMs with a water contact angle around 70°. In addition, a moderate hydrophilic surface (with a contact angle of 4090° for hMSCs and 70° for mMSCs) promoted osteogenic differentiation in the presence of biological stimuli. Hydrophilic mixed SAMs with a moderate wettability tended to promote the expression of αβ integrin of MSCs, indicating that the tunable wettability of the mixed SAMs may guide osteogenesis through mediating the αβ integrin signaling pathway. Our work can direct the design of biomaterials with controllable wettability to promote the adhesion, proliferation and differentiation of MSCs from different sources.
金表面的链烷硫醇自组装单分子层（SAMs）已被用作模型底物来研究表面化学对细胞行为的影响。然而，在研究干细胞命运时，很少有研究致力于具有可控润湿性的底物。在此，制备了混合的羟基（-OH）和甲基（-CH）封端的SAMs以形成具有不同润湿性的底物，用于研究润湿性对人和小鼠来源的间充质干细胞（MSCs）的黏附、铺展、增殖和成骨分化的影响。人胎儿MSCs（hMSCs）和小鼠骨髓MSCs（mMSCs）的黏附数量分别在水接触角为4070°和7090°的-OH/-CH混合SAMs上达到最大值。水接触角为2070°的亲水性混合SAMs也促进了hMSCs和mMSCs的铺展。hMSCs和mMSCs在水接触角约为70°的亲水性SAMs上增殖最为有利。此外，适度亲水的表面（hMSCs的接触角为4090°，mMSCs的接触角为70°）在存在生物刺激的情况下促进成骨分化。具有适度润湿性的亲水性混合SAMs倾向于促进MSCs的αβ整合素表达，表明混合SAMs的可调润湿性可能通过介导αβ整合素信号通路来引导成骨作用。我们的工作可以指导设计具有可控润湿性的生物材料，以促进不同来源MSCs的黏附、增殖和分化。
