Gleaves C A, Hursh D A, Meyers J D
Infectious Diseases References Laboratory, Chiles Research Institute, Providence Medical Center, Portland, Oregon 97213-2967.
J Clin Microbiol. 1992 Apr;30(4):1045-8. doi: 10.1128/jcm.30.4.1045-1048.1992.
The sensitivities of MRC-5 and mink lung (ML) cells in centrifugation culture were compared simultaneously for the detection of cytomegalovirus (CMV) IE antigen (immediate-early antigen) from clinical specimens. Of 413 samples assayed, 51 (12%) were positive for CMV by both centrifugation and standard cell culture. At 20 h postinoculation (p.i.), 46 of 51 (90.2%) CMV-positive specimens were detected in ML cells. At 40 h p.i., 50 of 51 (98.0%) CMV-positive specimens were detected in ML cells, compared with 48 of 51 (94.0%) in MRC-5 cells. There was no significant difference in the detection of CMV in either cell line by centrifugation culture. However, in 19 of 23 positive samples that had countable foci at 20 h p.i., there was a 25% increase in the number of positive foci observed for ML cells compared with MRC-5 cells. Less toxicity was also noted for ML cells than for MRC cells, particularly in viral blood specimens. These data suggest that ML cells are comparable to MRC-5 cells for the rapid detection of CMV by centrifugation culture.
同时比较了MRC-5细胞和貂肺（ML）细胞在离心培养中检测临床标本中巨细胞病毒（CMV）IE抗原（即刻早期抗原）的敏感性。在检测的413份样本中，51份（12%）通过离心培养和标准细胞培养均为CMV阳性。接种后20小时（p.i.），51份CMV阳性标本中有46份（90.2%）在ML细胞中被检测到。接种后40小时，51份CMV阳性标本中有50份（98.0%）在ML细胞中被检测到，而在MRC-5细胞中为51份中的48份（94.0%）。通过离心培养在两种细胞系中检测CMV没有显著差异。然而，在接种后20小时有可计数病灶的23份阳性样本中的19份中，与MRC-5细胞相比，ML细胞中观察到的阳性病灶数量增加了25%。还注意到ML细胞的毒性比MRC细胞小，特别是在病毒血液标本中。这些数据表明，ML细胞在通过离心培养快速检测CMV方面与MRC-5细胞相当。
