Li Yueyun, Han Jian, Chen Runhai, Ren Xiang, Wei Qin
School of Chemical Engineering, Shandong University of Technology, Zibo 255049, People's Republic of China.
School of Chemical Engineering, Shandong University of Technology, Zibo 255049, People's Republic of China.
Anal Biochem. 2015 Jan 15;469:76-82. doi: 10.1016/j.ab.2014.09.022. Epub 2014 Oct 18.
Prostate-specific antigen (PSA), as the specificity of prostate cancer markers, has been widely used in prostate cancer diagnosis and screening. In this study, we fabricated an electrochemical immunosensor for PSA detection using the amino-functionalized graphene sheet-ferrocenecarboxaldehyde composite materials (NH2-GS@FCA) and silver hybridized mesoporous silica nanoparticles (Ag@NH2-MCM48). Under optimal conditions, the fabricated immunosensor showed a wide linear range with PSA concentration (0.01-10.0ng·ml(-1)). Low detection limit (2pg·ml(-1)) proved the high sensitivity. In addition, the immunosensor possessed good stability and reproducibility. Moreover, the application to PSA analysis in serum samples yielded satisfactory results.
前列腺特异性抗原（PSA）作为前列腺癌的特异性标志物，已被广泛应用于前列腺癌的诊断和筛查。在本研究中，我们使用氨基功能化石墨烯片-二茂铁甲醛复合材料（NH2-GS@FCA）和银杂化介孔二氧化硅纳米颗粒（Ag@NH2-MCM48）制备了一种用于检测PSA的电化学免疫传感器。在最佳条件下，所制备的免疫传感器对PSA浓度（0.01-10.0ng·ml(-1)）呈现出较宽的线性范围。低检测限（2pg·ml(-1)）证明了其高灵敏度。此外，该免疫传感器具有良好的稳定性和重现性。而且，将其应用于血清样本中PSA的分析，结果令人满意。
