Bruce C B, al-Nakib W, Almond J W, Tyrrell D A
MRC Common Cold Unit, Salisbury, Wilts., U.K.
Arch Virol. 1989;105(3-4):179-87. doi: 10.1007/BF01311355.
Current methods of detecting a human rhinovirus (HRV) infection are either based on isolation of virus in appropriate susceptible cell lines, which is time-consuming and requires considerable expertise, or are dependent on knowing the serotype. The existence of over 100 immunologically distinct serotypes makes serotype specific assays, such as ELISA, unsuitable for general diagnostic assays. In this study a general rhinovirus assay is described which utilises synthetic oligonucleotides as probes in a filter hybridization assay. The probes are designed to bind to short but highly conserved regions of the rhinovirus genome. Indeed, the probes successfully detected all 57 rhinovirus serotypes tested. Furthermore, the test was used to demonstrate rhinovirus infection in clinical samples from 57 volunteers, inoculated with HRV, collected on six consecutive days. Clinical samples were taken prior to inoculation and on days 2-7 after inoculation. The filter hybridization assay gave results comparable to virus culture on days 2 and 3 post-inoculation, but was more sensitive on subsequent days.
目前检测人鼻病毒（HRV）感染的方法，要么是基于在合适的易感细胞系中分离病毒，这种方法耗时且需要相当专业的知识，要么依赖于已知血清型。超过100种免疫上不同的血清型的存在，使得血清型特异性检测方法，如酶联免疫吸附测定（ELISA），不适用于一般诊断检测。在本研究中，描述了一种通用的鼻病毒检测方法，该方法在滤膜杂交检测中使用合成寡核苷酸作为探针。这些探针被设计用于结合鼻病毒基因组中短但高度保守的区域。事实上，这些探针成功检测了所测试的所有57种鼻病毒血清型。此外，该检测方法还用于证明57名接种HRV的志愿者连续6天采集的临床样本中的鼻病毒感染情况。临床样本在接种前以及接种后第2至7天采集。滤膜杂交检测在接种后第2天和第3天给出的结果与病毒培养相当，但在随后几天更敏感。
