Forsyth M, al-Nakib W, Chadwick P, Stanway G, Hughes P J, Leckie G, Almond J W, Tyrrell D A
MRC Common Cold Unit, Salisbury, U.K.
Arch Virol. 1989;107(1-2):55-63. doi: 10.1007/BF01313878.
This study investigated the abilities of cDNA probes from the 5' and 3' ends of the genome of human rhinoviruses (HRV-) 14, 9, and 1B to detect RNA from 59 rhinovirus serotypes. The results show that probes from the 5' end of the genomes of HRV-14, 9, and 1 B detected a large number of serotypes but the detection rate was variable and depended on the degree of homology with the particular probe. In contrast, all the 3' end probes were specific for the homologous virus. However, a long HRV-9 probe detected a large number of serotypes. It was concluded that such cDNA probes would not detect all serotypes with equal efficiency. Synthetic oligonucleotides corresponding to short but highly conserved regions in the 5' non coding region may overcome this problem.
本研究调查了人鼻病毒（HRV-）14型、9型和1B型基因组5'端和3'端的cDNA探针检测59种鼻病毒血清型RNA的能力。结果表明，HRV-14型、9型和1B型基因组5'端的探针检测到大量血清型，但检测率各不相同，且取决于与特定探针的同源程度。相比之下，所有3'端探针都对同源病毒具有特异性。然而，一段较长的HRV-9探针检测到大量血清型。得出的结论是，此类cDNA探针不能以同等效率检测所有血清型。对应于5'非编码区短而高度保守区域的合成寡核苷酸可能会克服这一问题。
