Vivas Caio Vinicius, Moraes Ramiris César Souza, Alves-Araújo Anderson, Alves Marccus, Mariano-Neto Eduardo, van den Berg Cássio, Gaiotto Fernanda Amato
Departamento de Ciências Biológicas , Universidade Estadual de Santa Cruz , Ilhéus, BA , Brazil .
Departamento de Ciências Agrárias e Biológicas , Universidade Federal do Espírito Santo , São Mateus, ES , Brazil .
Genet Mol Biol. 2014 Oct;37(4):662-70. doi: 10.1590/S1415-47572014005000019. Epub 2014 Nov 3.
The Atlantic Forest is a phytogeographic domain with a high rate of endemism and large species diversity. The Sapotaceae is a botanical family for which species identification in the Atlantic Forest is difficult. An approach that facilitates species identification in the Sapotaceae is urgently needed because this family includes threatened species and valuable timber species. In this context, DNA barcoding could provide an important tool for identifying species in the Atlantic Forest. In this work, we evaluated four plant barcode markers (matK, rbcL, trnH-psbA and the nuclear ribosomal internal transcribed spacer region - ITS) in 80 samples from 26 species of Sapotaceae that occur in the Atlantic Forest. ITS yielded the highest average interspecific distance (0.122), followed by trnH-psbA (0.019), matK (0.008) and rbcL (0.002). For species discrimination, ITS provided the best results, followed by matK, trnH-psbA and rbcL. Furthermore, the combined analysis of two, three or four markers did not result in higher rates of discrimination than obtained with ITS alone. These results indicate that the ITS region is the best option for molecular identification of Sapotaceae species from the Atlantic Forest.
大西洋森林是一个特有率高且物种多样性丰富的植物地理区域。山榄科是一个在大西洋森林中难以进行物种鉴定的植物科。由于该科包含受威胁物种和珍贵木材物种，因此迫切需要一种便于山榄科物种鉴定的方法。在此背景下，DNA条形码可为大西洋森林中的物种鉴定提供重要工具。在这项研究中，我们对来自大西洋森林中26种山榄科植物的80个样本评估了四种植物条形码标记（matK、rbcL、trnH-psbA和核糖体DNA内转录间隔区——ITS）。ITS产生的平均种间距离最高（0.122），其次是trnH-psbA（0.019）、matK（0.008）和rbcL（0.002）。对于物种鉴别，ITS提供了最佳结果，其次是matK、trnH-psbA和rbcL。此外，两个、三个或四个标记的组合分析并未产生比单独使用ITS更高的鉴别率。这些结果表明，ITS区域是从大西洋森林中山榄科物种分子鉴定的最佳选择。
