Parry J V, Perry K R, Panday S, Mortimer P P
PHLS Virus Reference Laboratory, Central Public Health Laboratory, London, England.
J Med Virol. 1989 Aug;28(4):255-60. doi: 10.1002/jmv.1890280410.
The use of salivary samples to diagnose acute viral hepatitis was investigated. Tests for IgM antibody to hepatitis A virus (anti-HAV) on 29 acute-phase samples from serologically confirmed cases of hepatitis A were strongly reactive. Follow-up samples indicated that IgM anti-HAV persisted at moderate levels for 2-4 months and was not usually detectable thereafter. The ratio of IgM to IgG anti-HAV (RIA index) correlated closely with the interval from onset of infection. Significant levels of IgM anti-HAV were not detected in the saliva of 103 IgG anti-HAV positive and 102 IgG anti-HAV negative individuals nor of 4 individuals with hepatitis B. Similarly, IgM anti-HBc was present in the saliva of acute cases of hepatitis B, but not in the saliva of 25 IgG anti-HBc positive and 85 IgG anti-HBc negative individuals, nor of 24 individuals with recent hepatitis A. It is concluded that saliva is a convenient and satisfactory alternative to serum for the diagnosis of hepatitis A infection.
对使用唾液样本诊断急性病毒性肝炎进行了研究。对29份来自血清学确诊甲型肝炎病例的急性期样本进行甲型肝炎病毒IgM抗体（抗-HAV）检测，结果呈强阳性反应。随访样本显示，IgM抗-HAV在中等水平持续2至4个月，之后通常检测不到。IgM与IgG抗-HAV的比值（放射免疫分析指数）与感染开始后的间隔密切相关。在103名IgG抗-HAV阳性和102名IgG抗-HAV阴性个体以及4名乙型肝炎患者的唾液中均未检测到显著水平的IgM抗-HAV。同样，IgM抗-HBc存在于急性乙型肝炎病例的唾液中，但在25名IgG抗-HBc阳性和85名IgG抗-HBc阴性个体以及24名近期感染甲型肝炎的个体的唾液中未检测到。结论是，唾液是诊断甲型肝炎感染的一种方便且令人满意的替代血清的样本。
