Myers C, Frisque R J, Arthur R R
Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
J Virol. 1989 Oct;63(10):4445-9. doi: 10.1128/JVI.63.10.4445-4449.1989.
JC virus DNA was extracted from urine-derived cells of bone marrow and renal transplant patients and cloned directly into the plasmid vector pBR322. These clones represent the first JC virus isolates obtained directly from individuals that did not have progressive multifocal leukoencephalopathy (PML). Three of the clones appeared to be identical to the prototype JC virus Mad 1, and the fourth clone was identical to the type II JC virus variant Mad8-Br. Importantly, the same JC virus strains have been identified both in the urine of non-PML patients and in the brain tissue of PML patients. These results indicate that different organs may be infected with the same JC virus subtype and implies that an adaptation process involving the alteration of viral regulatory signals is not required in the pathogenesis of PML. Furthermore, both a type I and a type II variant were obtained from the same patient, suggesting that an individual may be infected with more than one strain of JC virus at a given time.
从骨髓和肾移植患者的尿液衍生细胞中提取 JC 病毒 DNA，并直接克隆到质粒载体 pBR322 中。这些克隆代表了首次直接从没有进行性多灶性白质脑病（PML）的个体中获得的 JC 病毒分离株。其中三个克隆似乎与原型 JC 病毒 Mad 1 相同，第四个克隆与 II 型 JC 病毒变体 Mad8-Br 相同。重要的是，在非 PML 患者的尿液和 PML 患者的脑组织中都鉴定出了相同的 JC 病毒株。这些结果表明，不同器官可能感染相同的 JC 病毒亚型，这意味着在 PML 的发病机制中不需要涉及病毒调节信号改变的适应过程。此外，从同一患者中获得了 I 型和 II 型变体，表明个体在给定时间可能感染不止一种 JC 病毒株。
