Vachali Preejith P, Li Binxing, Bartschi Alexis, Bernstein Paul S
Moran Eye Center, University of Utah School of Medicine, 65 Mario Capecchi Drive, Salt Lake City, UT 84132, United States.
Moran Eye Center, University of Utah School of Medicine, 65 Mario Capecchi Drive, Salt Lake City, UT 84132, United States.
Arch Biochem Biophys. 2015 Apr 15;572:66-72. doi: 10.1016/j.abb.2014.12.005. Epub 2014 Dec 13.
The surface plasmon resonance (SPR) biosensor method is a highly sensitive, label-free technique to study the non-covalent interactions of biomolecules, especially protein-protein and protein-small molecule interactions. We have explored this robust biosensor platform to study the interactions of carotenoid-binding proteins and their carotenoid ligands to assess the specificity of interaction, kinetics, affinity, and stoichiometry. These characterizations are important to further study uptake and transport of carotenoids to targeted tissues such as the macula of the human eye. In this review, we present an overview of the SPR method and optimization of assay conditions, and we discuss the particular challenges in studying carotenoid-protein interactions using SPR.
表面等离子体共振（SPR）生物传感器方法是一种高度灵敏的无标记技术，用于研究生物分子的非共价相互作用，特别是蛋白质-蛋白质和蛋白质-小分子相互作用。我们探索了这个强大的生物传感器平台，以研究类胡萝卜素结合蛋白与其类胡萝卜素配体之间的相互作用，从而评估相互作用的特异性、动力学、亲和力和化学计量。这些表征对于进一步研究类胡萝卜素向人眼黄斑等靶组织的摄取和转运非常重要。在这篇综述中，我们概述了SPR方法和检测条件的优化，并讨论了使用SPR研究类胡萝卜素-蛋白质相互作用时的特殊挑战。
