March Rosselló Gabriel Alberto, Bratos Pérez Miguel Ángel
Departamento de Microbiología, Facultad de Medicina, Universidad de Valladolid, Valladolid, España.
Departamento de Microbiología, Facultad de Medicina, Universidad de Valladolid, Valladolid, España; Servicio de Microbiología, Hospital Clínico Universitario de Valladolid, Valladolid, España.
Enferm Infecc Microbiol Clin. 2016 Jan;34(1):61-8. doi: 10.1016/j.eimc.2014.11.014. Epub 2015 Jan 2.
The most widely used antibiotic susceptibility testing methods in Clinical Microbiology are based on the phenotypic detection of antibiotic resistance by measuring bacterial growth in the presence of the antibiotic being tested. These conventional methods take typically 24hours to obtain results. A review is presented here of recently developed techniques for the rapid determination of antibiotic susceptibility. Data obtained with different methods such as molecular techniques, flow cytometry, chemiluminescence, mass spectrometry, commercial methods used in routine work, colorimetric methods, nephelometry, microarrays, microfluids, and methods based on cell disruption and sequencing, are analyzed and discussed in detail.
临床微生物学中使用最广泛的抗生素敏感性测试方法是基于通过测量在被测抗生素存在下细菌的生长来对抗生素耐药性进行表型检测。这些传统方法通常需要24小时才能获得结果。本文综述了最近开发的用于快速测定抗生素敏感性的技术。详细分析和讨论了用不同方法获得的数据，如分子技术、流式细胞术、化学发光、质谱、常规工作中使用的商业方法、比色法、散射比浊法、微阵列、微流体以及基于细胞破碎和测序的方法。
