March-Rosselló Gabriel Alberto
Servicio de Microbiología e Inmunología, Hospital Clínico Universitario de Valladolid, Valladolid, España.
Enferm Infecc Microbiol Clin. 2017 Mar;35(3):182-188. doi: 10.1016/j.eimc.2016.12.005. Epub 2017 Jan 18.
The most widely used antibiotic susceptibility testing methods in Clinical Microbiology are based on the phenotypic detection of antibiotic resistance by measuring bacterial growth in the presence of the antibiotic being tested. These conventional methods take typically 24hours to obtain results. Here we review the main techniques for rapid determination of antibiotic susceptibility. Data obtained with different methods such as molecular techniques, microarrays, commercial methods used in work routine, immunochromatographic methods, colorimetric methods, image methods, nephelometry, MALDI-TOF mass spectrometry, flow cytometry, chemiluminescence and bioluminescence, microfluids and methods based on cell disruption are analysed in detail.
临床微生物学中使用最广泛的抗生素敏感性测试方法是基于通过测量在受试抗生素存在下的细菌生长来进行抗生素耐药性的表型检测。这些传统方法通常需要24小时才能获得结果。在此，我们综述了快速测定抗生素敏感性的主要技术。详细分析了用不同方法获得的数据，如分子技术、微阵列、日常工作中使用的商业方法、免疫色谱法、比色法、图像法、散射比浊法、基质辅助激光解吸电离飞行时间质谱法、流式细胞术、化学发光和生物发光法、微流体法以及基于细胞裂解的方法。
