Liu Tingting, Qi He, Ma Long, Liu Zhaojun, Fu Huiling, Zhu Wenzhen, Song Taiyu, Yang Bingwu, Li Guorong
Shandong Provincial Key Laboratory of Animal Resistant Biology, School of Life Sciences, Shandong Normal University , Jinan, China .
Rejuvenation Res. 2015 Jun;18(3):225-33. doi: 10.1089/rej.2014.1618. Epub 2015 May 20.
Resveratrol is a natural polyphenol derived mainly from the skin of grapes and from red wine. Resveratrol prolongs life span in several invertebrates, but this function is not found in mice. Our recently published paper demonstrated that resveratrol prolonged longevity of the annual fish Nothobranchius guentheri, a promising vertebrate model for anti-aging research. However, the anti-aging process by resveratrol remains largely unexplored, and little is known about its effects on oxidative stress. In this study, by long-term supplementation of resveratrol from sexual maturity onward in the annual fish, we detected survivorship and oxidative stress at three different developmental stages in vivo. A total of 112 fish were fed with resveratrol in the concentration of 200 μg/gram food and 111 fish without resveratrol from 16 weeks of age until to the end of their lives. The mean and maximum life spans of the fish treated with resveratrol were extended by 17.34% and 17.66%, respectively, compared to the fish in control group. The markers of oxidative stress, such as the levels of reactive oxygen species (ROS), the activities of anti-oxidant enzymes, and the degree of oxidative damage, were detected at 6, 9, and 12 months, respectively. The results showed that levels of ROS and oxidative damage increased and activities of anti-oxidant enzymes appeared to decrease with age. Resveratrol treatment significantly attenuated the increase of ROS and oxidative damage and up-regulated the decrease of anti-oxidant enzyme activities induced by aging. Our results demonstrated that resveratrol decreased oxidative stress and extended life span in this short-lived fish.
白藜芦醇是一种天然多酚，主要来源于葡萄皮和红酒。白藜芦醇可延长几种无脊椎动物的寿命，但在小鼠中未发现此功能。我们最近发表的论文表明，白藜芦醇可延长一年生鱼类冈氏假鳃鳉的寿命，这是一种用于抗衰老研究的有前景的脊椎动物模型。然而，白藜芦醇的抗衰老过程在很大程度上仍未被探索，其对氧化应激的影响也知之甚少。在本研究中，通过从一年生鱼类性成熟开始长期补充白藜芦醇，我们在体内三个不同发育阶段检测了存活率和氧化应激。从16周龄直至生命结束，总共112条鱼被喂食浓度为200μg/克食物的白藜芦醇，111条鱼未喂食白藜芦醇。与对照组的鱼相比，用白藜芦醇处理的鱼的平均寿命和最长寿命分别延长了17.34%和17.66%。分别在6个月、9个月和12个月时检测氧化应激标志物，如活性氧（ROS）水平、抗氧化酶活性和氧化损伤程度。结果表明，ROS水平和氧化损伤随年龄增加，抗氧化酶活性似乎随年龄降低。白藜芦醇处理显著减弱了ROS和氧化损伤的增加，并上调了衰老诱导的抗氧化酶活性的降低。我们的结果表明，白藜芦醇可降低这种短命鱼类的氧化应激并延长其寿命。
