Shehab Naglaa Gamil, Abu-Gharbieh Eman
Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Giza, Egypt.
Department of Pharmacology and Toxicology, Dubai Pharmacy College, P.O. Box 19099, Dubai, UAE.
Evid Based Complement Alternat Med. 2014;2014:695291. doi: 10.1155/2014/695291. Epub 2014 Dec 21.
Reported researches dealing with either composition or bioactivity of Salsola imbricata are limited. This study was conducted aiming to investigate the phenolic composition of the plant and evaluate its efficacy as male contraceptive. Polyphenols, namely, phenolic acids and flavonoids, were qualitatively and quantitatively analysed by RP-HPLC in the hydrolysed methanol extract using two different wavelengths, 280 and 330 nm. The efficiency of different solvents in extracting the plant phenolics was assessed via spectrophotometric determination of the total phenolic and flavonoid contents. Acute toxicity study was carried out on the ethanolic extract to ascertain its safety prior to biological evaluation. The contraceptive effect was assessed, in male rats, by oral administration of the extract at two doses (250 and 500 mg/kg b. wt.), over a period of 65 days. HPLC analyses allowed the identification and quantification of a total of 13 and 8 components in the hydrolysed-methanol extract; the overall phenolic composition was dominated by quercitrin (12.692%) followed by coumaric acid (4.251%). Prolonged oral administration of the ethanolic extract caused slight reduction in the testis weight only. A significant decrease in the sperm count was observed (P < 0.01) in the two treated groups while significant decrease in the epididymal sperm motility was only observed in the high dose group. Morphological abnormalities were observed in sperms of treated animals. No distinct change in serum FSH, LH, and testosterone concentration was recorded. The histopathological findings supported to a high extent these results. The male contraceptive activity of Salsola imbricata could be ascribed to its phenolic components, especially quercitrin.
关于翅碱蓬的成分或生物活性的已报道研究有限。本研究旨在调查该植物的酚类成分并评估其作为男性避孕药的功效。使用280和330nm这两个不同波长，通过RP-HPLC对水解甲醇提取物中的多酚（即酚酸和黄酮类化合物）进行定性和定量分析。通过分光光度法测定总酚和黄酮含量来评估不同溶剂提取植物酚类的效率。在进行生物学评估之前，对乙醇提取物进行急性毒性研究以确定其安全性。在雄性大鼠中，通过口服给予两种剂量（250和500mg/kg体重）的提取物，持续65天来评估避孕效果。HPLC分析可鉴定和定量水解甲醇提取物中的总共13种和8种成分；总体酚类成分以槲皮苷（12.692%）为主，其次是香豆酸（4.251%）。长期口服乙醇提取物仅导致睾丸重量略有下降。在两个治疗组中观察到精子数量显著减少（P<0.01），而仅在高剂量组中观察到附睾精子活力显著下降。在处理动物的精子中观察到形态异常。血清FSH、LH和睾酮浓度未记录到明显变化。组织病理学结果在很大程度上支持了这些结果。翅碱蓬的男性避孕活性可能归因于其酚类成分，尤其是槲皮苷。
