Center for Nanointegration Duisburg-Essen (CENIDE) and Center for Medical Biotechnology (ZMB), University of Duisburg-Essen, Universitätsstrasse 2, 45117 Essen (Germany).
Angew Chem Int Ed Engl. 2015 Mar 16;54(12):3592-7. doi: 10.1002/anie.201408941. Epub 2015 Jan 28.
Today, DNA nanotechnology is one of the methods of choice to achieve spatiotemporal control of matter at the nanoscale. By combining the peculiar spatial addressability of DNA origami structures with the switchable mechanical movement of small DNA motifs, we constructed reconfigurable DNA nanochambers as dynamic compartmentalization systems. The reversible extension and contraction of the inner cavity of the structures was used to control the distance-dependent energy transfer between two preloaded fluorophores. Interestingly, single-molecule FRET studies revealed that the kinetics of the process are strongly affected by the choice of the switchable motifs and/or actuator sequences, thus offering a valid method for fine-tuning the dynamic properties of large DNA nanostructures. We envisage that the proposed DNA nanochambers may function as model structures for artificial biomimetic compartments and transport systems.
如今，DNA 纳米技术是实现纳米尺度物质时空控制的首选方法之一。通过将 DNA 折纸结构的特殊空间寻址能力与小分子 DNA 图案的可切换机械运动相结合，我们构建了可重构的 DNA 纳米室作为动态分隔系统。结构内部腔的可逆扩展和收缩用于控制两个预加载荧光团之间的距离相关能量转移。有趣的是，单分子 FRET 研究表明，该过程的动力学强烈受到可切换图案和/或致动器序列的选择的影响，从而为精细调整大型 DNA 纳米结构的动态特性提供了一种有效方法。我们设想，所提出的 DNA 纳米室可以作为人工仿生隔间和运输系统的模型结构。
