Kopf Brigitte S, Ruch Sylvie, Berner Simon, Spencer Nicholas D, Maniura-Weber Katharina
Department Materials meet Life, Laboratory for Materials Biology Interactions, Empa, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen, Switzerland.
Institut Straumann AG, Basel, Switzerland.
J Biomed Mater Res A. 2015 Aug;103(8):2661-72. doi: 10.1002/jbm.a.35401. Epub 2015 Feb 11.
Protein adsorption and blood coagulation play important roles in the early stages of osseointegration and are strongly influenced by surface properties. We present a systematic investigation of the influence of different surface properties on the adsorption of the blood proteins fibrinogen and fibronectin and the degree of early blood coagulation. Experiments on custom-made and commercially available, microroughened hydrophobic titanium (Ti) surfaces (Ti SLA-Hphob ), hydrophilic (Hphil ) microroughened Ti surfaces with nanostructures (Ti SLActive-Hphil NS), and on bimetallic Ti zirconium alloy (TiZr, Roxolid®) samples were performed, to study the biological response in relation to the surface wettability and the presence of nanostructures (NS). Protein adsorption on the different substrates showed a highly significant effect of surface NS. Hydrophilicity alone did not significantly enhance protein adsorption. Overall, the combination of NS and hydrophilicity led to the highest adsorption levels; independent of whether Ti or TiZr were used. Hydrophilicity induced a strong effect on blood coagulation, whereas the effect of NS alone was weak. The combination of both surface characteristics led to early and most pronounced blood-coagulation. Therefore, nanostructured, hydrophilic Ti and TiZr surfaces may perform better in terms of osseointegration due to continuous protein adsorption and the formation of a layer of blood components on the implant surface.
蛋白质吸附和血液凝固在骨整合的早期阶段起着重要作用，并且受到表面性质的强烈影响。我们对不同表面性质对血液蛋白纤维蛋白原和纤连蛋白吸附以及早期血液凝固程度的影响进行了系统研究。在定制的和市售的、具有微粗糙度的疏水钛（Ti）表面（Ti SLA-Hphob）、具有纳米结构的亲水性（Hphil）微粗糙Ti表面（Ti SLActive-Hphil NS）以及双金属钛锆合金（TiZr，Roxolid®）样品上进行了实验，以研究与表面润湿性和纳米结构（NS）的存在相关的生物学反应。不同底物上的蛋白质吸附显示出表面NS的高度显著影响。单独的亲水性并没有显著增强蛋白质吸附。总体而言，NS和亲水性的组合导致了最高的吸附水平；无论使用的是Ti还是TiZr。亲水性对血液凝固有强烈影响，而单独的NS影响较弱。两种表面特性的组合导致早期且最明显的血液凝固。因此，由于在植入物表面持续的蛋白质吸附和血液成分层的形成，纳米结构的亲水性Ti和TiZr表面在骨整合方面可能表现得更好。
