Department of Bioengineering, University of California, Los Angeles , Los Angeles, California 90095, USA.
Biomicrofluidics. 2015 Feb 5;9(1):014112. doi: 10.1063/1.4907807. eCollection 2015 Jan.
Exosomes, nanosized membrane-bound vesicles released by cells, play roles in cell signaling, immunology, virology, and oncology. Their study, however, has been hampered by difficulty in isolation and quantification due to their size and the complexity of biological samples. Conventional approaches to improved isolation require specialized equipment and extensive sample preparation time. Therefore, isolation and detection methods of exosomes will benefit biological and clinical studies. Here, we report a microfluidic platform for inline exosome isolation and fluorescent detection using inertial manipulation of antibody-coated exosome capture beads from biological fluids.
细胞释放的纳米大小的膜结合囊泡外泌体在细胞信号转导、免疫学、病毒学和肿瘤学中发挥作用。然而，由于其大小和生物样本的复杂性，其分离和定量一直受到阻碍。改进分离的传统方法需要专门的设备和广泛的样品制备时间。因此，外泌体的分离和检测方法将有利于生物和临床研究。在这里，我们报告了一种使用基于惯性的微流控平台，从生物流体中对抗体包被的外泌体捕获珠进行在线外泌体分离和荧光检测。
