Horii Y, Muraguchi A, Iwano M, Matsuda T, Hirayama T, Yamada H, Fujii Y, Dohi K, Ishikawa H, Ohmoto Y
Division of Immunology, Osaka University, Japan.
J Immunol. 1989 Dec 15;143(12):3949-55.
In this study, we demonstrated that IL-6 was a possible autocrine growth factor for rat mesangial cells (MC). rIL-6 induced in vitro growth of rat MC at a concentration of 2 to 200 ng/ml and IL-6 activity was found in the supernatant of cultured rat MC. Northern blot analysis as well as in situ hybridization revealed that IL-6 mRNA was expressed in the cultured MC. Of urine samples from patients with mesangial proliferative glomerulonephritis (PGN) 50% were found to contain significant IL-6 activity (ranging from 30 to 126 pg/ml). Urine samples from other type of primary glomerular diseases such as minimal change nephrotic syndrome or healthy volunteers contained no detectable IL-6 activity. Only 2 of 27 urine samples from membranous nephropathy contained detectable amount of IL-6. Furthermore, there was some relationship between the levels of urine IL-6 and the progressive stage of PGN. Finally, by immunohistochemical staining using an anti-IL-6 mAb, it was shown that MC in the affected glomeruli of PGN patients produced IL-6, whereas MC obtained from the patients with membranous nephropathy, minimal change nephrotic syndrome or normal kidney were not found to produce IL-6. These data suggest that deregulated production of IL-6 is involved in PGN and the measurement of urine IL-6 is helpful for the differential diagnosis of PGN as well as for monitoring the progression of PGN.
在本研究中，我们证明白细胞介素-6（IL-6）可能是大鼠系膜细胞（MC）的自分泌生长因子。重组IL-6在浓度为2至200 ng/ml时可诱导大鼠MC的体外生长，并且在培养的大鼠MC上清液中发现了IL-6活性。Northern印迹分析以及原位杂交显示，培养的MC中表达了IL-6 mRNA。在系膜增生性肾小球肾炎（PGN）患者的尿液样本中，发现50%含有显著的IL-6活性（范围为30至126 pg/ml）。其他类型的原发性肾小球疾病（如微小病变肾病综合征）患者的尿液样本或健康志愿者的尿液样本中未检测到IL-6活性。膜性肾病的27份尿液样本中只有2份含有可检测量的IL-6。此外，尿液IL-6水平与PGN的进展阶段之间存在一定关系。最后，通过使用抗IL-6单克隆抗体的免疫组织化学染色显示，PGN患者受影响肾小球中的MC产生IL-6，而从膜性肾病、微小病变肾病综合征患者或正常肾脏获取的MC未发现产生IL-6。这些数据表明，IL-6的失控产生与PGN有关，尿液IL-6的检测有助于PGN的鉴别诊断以及监测PGN的进展。
