Eteraf-Oskouei Tahereh, Shafiee-Khamneh Ayda, Heshmati-Afshar Fariba, Delazar Abbas
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, I.R. Iran.
Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, I.R. Iran.
Res Pharm Sci. 2020 Feb 20;15(1):66-75. doi: 10.4103/1735-5362.278716. eCollection 2020 Feb.
Research on new drugs with a natural source and low side effects is a priority in pharmacology studies. The present study was conducted to investigate the anti-inflammatory and anti-angiogenesis effects of bee pollen extract in the air pouch model of inflammation.
To achieve this goal, male rats were moderately anesthetized and then 20 and 10 mL of sterile air were subcutaneously injected into the intrascapular area of the back of the rat on first and third days, respectively. On day 6, inflammation was induced by intrapouch injection of carrageenan. Normal saline in the control group and bee pollen methanolic extract (50, 100, and 200 mg/pouch) were administered at day 6, simultaneously with carrageenan, and then for 2 consecutive days only normal saline and the extracts were injected. Following sacrificing the rats the pouch was opened and the exudate volume, leukocyte accumulation, granulation tissue weight, vascular endothelial growth factor (VEGF), interleukin 1beta, and tumor necrosis factor alpha (TNF-α) concentrations were determined 3 days after induction of inflammation. In order to investigate the angiogenesis, the granulation tissue was removed, homogenized in the Drabkin's reagent, and then centrifuged. The supernatant was filtered and the hemoglobin concentration was determined using a spectrophotometer.
Bee pollen extract significantly decreased the exudate volume, leukocyte accumulation, granulation tissue weight, angiogenesis, VEGF, and TNF-α concentration.
The findings of the current study revealed that bee pollen methanolic extract has an anti-inflammatory and anti-angiogenesis effect, which could be attributed to the inhibition of VEGF and TNF-α production in the inflammatory exudates.
研发具有天然来源且副作用低的新药是药理学研究的重点。本研究旨在探讨蜂花粉提取物在炎症气袋模型中的抗炎和抗血管生成作用。
为实现这一目标，将雄性大鼠适度麻醉，然后分别在第一天和第三天于大鼠背部肩胛间区域皮下注射20 mL和10 mL无菌空气。在第6天，通过向气袋内注射角叉菜胶诱导炎症。对照组注射生理盐水，在第6天与角叉菜胶同时给予蜂花粉甲醇提取物（50、100和200 mg/气袋），然后仅连续2天注射生理盐水和提取物。处死大鼠后，打开气袋，在炎症诱导3天后测定渗出液体积、白细胞积聚、肉芽组织重量、血管内皮生长因子（VEGF）、白细胞介素1β和肿瘤坏死因子α（TNF-α）浓度。为研究血管生成，取出肉芽组织，在血红蛋白试剂中匀浆，然后离心。过滤上清液，用分光光度计测定血红蛋白浓度。
蜂花粉提取物显著降低了渗出液体积、白细胞积聚、肉芽组织重量、血管生成、VEGF和TNF-α浓度。
本研究结果表明，蜂花粉甲醇提取物具有抗炎和抗血管生成作用，这可能归因于其对炎症渗出物中VEGF和TNF-α产生的抑制作用。
