Department of Plant Sciences, University of Oxford , Oxford OX1 3RB, UK.
Centre for Immunity, Infection and Evolution, Ashworth Laboratories, University of Edinburgh , Edinburgh EH9 3JT, UK.
Sci Data. 2014 Aug 5;1:140024. doi: 10.1038/sdata.2014.24. eCollection 2014.
The availability of genome sequence data has greatly enhanced our understanding of the adaptations of trypanosomatid parasites to their respective host environments. However, these studies remain somewhat restricted by modest taxon sampling, generally due to focus on the most important pathogens of humans. To address this problem, at least in part, we are releasing a draft genome sequence for the African crocodilian trypanosome, Trypanosoma grayi ANR4. This dataset comprises genomic DNA sequences assembled de novo into contigs, encompassing over 10,000 annotated putative open reading frames and predicted protein products. Using phylogenomic approaches we demonstrate that T. grayi is more closely related to Trypanosoma cruzi than it is to the African trypanosomes T. brucei, T. congolense and T. vivax, despite the fact T. grayi and the African trypanosomes are each transmitted by tsetse flies. The data are deposited in publicly accessible repositories where we hope they will prove useful to the community in evolutionary studies of the trypanosomatids.
基因组序列数据的可用性极大地增强了我们对原生动物寄生虫适应各自宿主环境的理解。然而，这些研究仍然受到分类采样的限制，这主要是由于研究重点集中在人类最重要的病原体上。为了解决这个问题，我们至少部分地发布了非洲鳄原生动物锥虫 Trypanosoma grayi ANR4 的基因组草案序列。该数据集由从头组装的基因组 DNA 序列组成，包含超过 10000 个注释的假定开放阅读框和预测的蛋白质产物。使用系统基因组学方法，我们证明 T. grayi 与 Trypanosoma cruzi 的亲缘关系比与非洲锥虫 T. brucei、T. congolense 和 T. vivax 的亲缘关系更密切，尽管 T. grayi 和非洲锥虫都是由采采蝇传播的。这些数据已存入公共可访问的存储库，我们希望它们能对进化研究原生动物寄生虫的社区有用。
