用于酵母自噬相关蛋白的荧光工具集。
A fluorescent tool set for yeast Atg proteins.
作者信息
Li Dan, Song Jing-Zhen, Shan Mei-Hua, Li Shi-Ping, Liu Wei, Li Hui, Zhu Jing, Wang Yue, Lin Jianping, Xie Zhiping
机构信息
a School of Life Sciences and Biotechnology; Shanghai Jiao Tong University ; Shanghai , China.
出版信息
Autophagy. 2015;11(6):954-60. doi: 10.1080/15548627.2015.1040971.
Abstract
Fluorescence microscopy of live cells is instrumental in deciphering the molecular details of autophagy. To facilitate the routine examination of yeast Atg proteins under diverse conditions, here we provide a comprehensive tool set, including (1) plasmids for the expression of GFP chimeras at endogenous levels for most Atg proteins, (2) RFP-Atg8 constructs with improved properties as a PAS marker, and (3) plasmids for the complementation of common yeast auxotrophic markers. We hope that the availability of this tool set will further accelerate yeast autophagy research.
摘要
活细胞荧光显微镜检查有助于解读自噬的分子细节。为便于在不同条件下对酵母自噬相关蛋白（Atg蛋白）进行常规检测，我们在此提供一套全面的工具集，包括：（1）用于在大多数Atg蛋白的内源性水平表达绿色荧光蛋白（GFP）嵌合体的质粒；（2）作为吞噬泡前体结构（PAS）标记物、性能得到改进的红色荧光蛋白（RFP）-Atg8构建体；（3）用于补充常见酵母营养缺陷型标记物的质粒。我们希望这套工具集的问世将进一步加速酵母自噬研究。
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