对 Nostochopsis lobatus MAC0804NAN 中具有高透明质酸酶抑制活性的多糖进行分离与分析。

Isolation and analysis of polysaccharide showing high hyaluronidase inhibitory activity in Nostochopsis lobatus MAC0804NAN.

作者信息

Yamaguchi Yuji, Koketsu Mamoru

机构信息

Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan; MAC Gifu Research Institute, MicroAlgae Corporation, 4-15 Akebono, Gifu 500-8148, Japan.

Department of Chemistry and Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan.

出版信息

J Biosci Bioeng. 2016 Mar;121(3):345-8. doi: 10.1016/j.jbiosc.2015.07.008. Epub 2015 Aug 18.

DOI:10.1016/j.jbiosc.2015.07.008

PMID:26296532

Abstract

An active substance with high hyaluronidase inhibitory effect was isolated from the edible cyanobacterium Nostochopsis lobatus MAC0804NAN strain and characterized. The active component in the hot water extract was purified by anion exchange and gel filtration chromatography and was found to be a polysaccharide. The IC(50) against hyaluronidase of the purified polysaccharide was 7.18 μg/ml whose inhibitory activity is 14.5 times stronger than that of disodium cromoglycate (DSCG), an anti-allergy medication. The carbohydrate composition which was analyzed by GC-MS and NMR was found to be composed mainly of glucose, glucuronic acid, fucose, 2-O-methylfucose, mannose, galactose and xylose.

摘要

从可食用蓝藻念珠藻Nostochopsis lobatus MAC0804NAN菌株中分离并鉴定出一种具有高透明质酸酶抑制作用的活性物质。热水提取物中的活性成分通过阴离子交换和凝胶过滤色谱法进行纯化，结果发现是一种多糖。纯化后的多糖对透明质酸酶的IC(50)为7.18 μg/ml，其抑制活性比抗过敏药物色甘酸钠（DSCG）强14.5倍。通过气相色谱-质谱联用（GC-MS）和核磁共振（NMR）分析的碳水化合物组成主要由葡萄糖、葡萄糖醛酸、岩藻糖、2-O-甲基岩藻糖、甘露糖、半乳糖和木糖组成。