Sonker Atul, Dubey Anju, Bhatnagar Ankur, Chaudhary Rajendra
Department of Transfusion Medicine, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, Uttar Pradesh, India.
Department of Transfusion Medicine, All India Institute of Medical Sciences, Rishikesh, Uttarakhand, India.
Asian J Transfus Sci. 2015 Jul-Dec;9(2):155-8. doi: 10.4103/0973-6247.162712.
Platelets are a source of numerous growth factors which facilitate repair and healing. Thus platelet rich plasma has been increasingly used as a treatment modality in the field of reconstructive surgeries for wound healing. This preliminary study was carried out to explore whether platelet growth factors from platelet rich plasma could be used for enhancement of split thickness skin graft survival.
Twenty patients (13 males and 7 females) requiring split thickness skin graft for various clinical reasons were enrolled in the study. Platelet rich plasma was collected by apheresis and frozen at -80° C. It was thawed at room temperature immediately before its intended application. PRP was applied only on one half of the wound, while another half served as control. Patient was followed for 6 weeks. The effect was assessed at first dressing in terms of graft uptake and subsequently as time taken for complete healing.
There was 100% uptake of the graft in the area where platelet rich plasma was applied. In the control area, there was complete graft loss in 4 cases, partial loss in 7 cases and complete uptake in 9 cases.
This study demonstrated promising results on application of PRP to split thickness skin grafts. Further randomized studies with greater sample size may be undertaken to establish platelet rich plasma as a validated treatment modality.
血小板是多种生长因子的来源，这些生长因子有助于修复和愈合。因此，富血小板血浆越来越多地被用作重建手术领域中促进伤口愈合的一种治疗方式。本初步研究旨在探讨富血小板血浆中的血小板生长因子是否可用于提高中厚皮片的存活率。
本研究纳入了20例因各种临床原因需要进行中厚皮片移植的患者（13例男性和7例女性）。通过血液成分单采术收集富血小板血浆，并在-80°C下冷冻。在即将使用前，将其在室温下解冻。富血小板血浆仅应用于伤口的一半，而另一半作为对照。对患者进行6周的随访。在首次换药时根据皮片摄取情况评估效果，随后评估完全愈合所需的时间。
在应用富血小板血浆的区域，皮片摄取率为100%。在对照区域，4例皮片完全丢失，7例部分丢失，9例完全被摄取。
本研究表明将富血小板血浆应用于中厚皮片取得了有前景的结果。可能需要进行进一步的大样本随机研究，以确立富血小板血浆作为一种有效的治疗方式。
