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猪肠道微生物群对肠炎沙门氏菌和胞内劳森菌感染的反应变化

Changes in the Porcine Intestinal Microbiome in Response to Infection with Salmonella enterica and Lawsonia intracellularis.

作者信息

Borewicz Klaudyna A, Kim Hyeun Bum, Singer Randall S, Gebhart Connie J, Sreevatsan Srinand, Johnson Timothy, Isaacson Richard E

机构信息

Department of Veterinary and Biomedical Sciences, University of Minnesota, 1971 Commonwealth Ave., St. Paul, MN, 55108, United States of America.

出版信息

PLoS One. 2015 Oct 13;10(10):e0139106. doi: 10.1371/journal.pone.0139106. eCollection 2015.

Abstract

Salmonella enterica is a leading cause of food borne illness. Recent studies have shown that S. enterica is a pathogen capable of causing alterations to the composition of the intestinal microbiome. A recent prospective study of French pork production farms found a statistically significant association between Lawsonia intracellularis and carriage of S. enterica. In the current study the composition of the gut microbiome was determined in pigs challenged with S. enterica serovar Typhimurium and or L. intracellularis and compared to non-challenged control pigs. Principal coordinate analysis demonstrated that there was a disruption in the composition of the gut microbiome in the colon and cecum of pigs challenged with either pathogen. The compositions of the microbiomes of challenged pigs were similar to each other but differed from the non-challenged controls. There also were statistically significant increases in Anaerobacter, Barnesiella, Pediococcus, Sporacetigenium, Turicibacter, Catenibacterium, Prevotella, Pseudobutyrivibrio, and Xylanibacter in the challenged pigs. To determine if these changes were specific to experimentally challenged pigs, we determined the compositions of the fecal microbiomes of naturally infected pigs that were carriers of S. enterica. Pigs that were frequent shedders of S. enterica were shown to have similar fecal microbiomes compared to non-shedders or pigs that shed S. enterica infrequently. In a comparison of the differentially abundant bacteria in the naturally infected pigs compared to experimentally challenged pigs, 9 genera were differentially abundant and each exhibited the same increase or decrease in abundance between the two groups. Thus, there were similar changes in the GI microbiome associated with carriage of S. enterica regardless of whether the pigs were experimentally challenged with S. enterica or acquired it naturally.

摘要

肠炎沙门氏菌是食源性疾病的主要病因。最近的研究表明,肠炎沙门氏菌是一种能够改变肠道微生物群组成的病原体。最近一项针对法国猪肉生产农场的前瞻性研究发现,胞内劳森菌与肠炎沙门氏菌的携带之间存在统计学上的显著关联。在本研究中,测定了用肠炎沙门氏菌血清型鼠伤寒沙门氏菌和/或胞内劳森菌攻击的猪的肠道微生物群组成,并与未攻击的对照猪进行比较。主坐标分析表明,用任何一种病原体攻击的猪的结肠和盲肠中,肠道微生物群的组成都受到了破坏。受攻击猪的微生物群组成彼此相似,但与未受攻击的对照猪不同。受攻击猪中的厌氧杆菌属(Anaerobacter)、巴恩斯氏菌属(Barnesiella)、片球菌属(Pediococcus)、产孢梭菌属(Sporacetigenium)、Turicibacter、链状杆菌属(Catenibacterium)、普雷沃氏菌属(Prevotella)、假丁酸弧菌属(Pseudobutyrivibrio)和木聚糖杆菌属(Xylanibacter)也有统计学上的显著增加。为了确定这些变化是否特定于实验性攻击的猪,我们测定了作为肠炎沙门氏菌携带者的自然感染猪的粪便微生物群组成。结果显示,与非排菌猪或偶尔排菌的猪相比,频繁排肠炎沙门氏菌的猪具有相似的粪便微生物群。在比较自然感染猪与实验性攻击猪中差异丰富的细菌时,有9个属差异丰富,并且在两组之间每个属的丰度都呈现相同的增加或减少。因此,无论猪是通过实验性感染肠炎沙门氏菌还是自然感染,与肠炎沙门氏菌携带相关的胃肠道微生物群都有相似的变化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ce5b/4604083/25a6bc9bc124/pone.0139106.g001.jpg

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