Dong Yumei, Su Yuan, Yu Ping, Yang Min, Zhu Shusheng, Mei Xinyue, He Xiahong, Pan Manhua, Zhu Youyong, Li Chengyun
Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, China.
The Life Science and Technology Department of Kunming University, Kunming, 650214, China.
PLoS One. 2015 Oct 29;10(10):e0141264. doi: 10.1371/journal.pone.0141264. eCollection 2015.
Nonhost resistance (NHR) pertains to the most common form of plant resistance against pathogenic microorganisms of other species. Bipolaris maydis is a non-adapted pathogen affecting soybeans, particularly of maize/soybean intercropping systems. However, no experimental evidence has described the immune response of soybeans against B. maydis. To elucidate the molecular mechanism underlying NHR in soybeans, proteomics analysis based on two-dimensional polyacrylamide gel electrophoresis (2-DE) was performed to identify proteins involved in the soybean response to B. maydis. The spread of B. maydis spores across soybean leaves induced NHR throughout the plant, which mobilized almost all organelles and various metabolic processes in response to B. maydis. Some enzymes, including ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), mitochondrial processing peptidase (MPP), oxygen evolving enhancer (OEE), and nucleoside diphosphate kinase (NDKs), were found to be related to NHR in soybeans. These enzymes have been identified in previous studies, and STRING analysis showed that most of the protein functions related to major metabolic processes were induced as a response to B. maydis, which suggested an array of complex interactions between soybeans and B. maydis. These findings suggest a systematic NHR against non-adapted pathogens in soybeans. This response was characterized by an overlap between metabolic processes and response to stimulus. Several metabolic processes provide the soybean with innate immunity to the non-adapted pathogen, B. maydis. This research investigation on NHR in soybeans may foster a better understanding of plant innate immunity, as well as the interactions between plant and non-adapted pathogens in intercropping systems.
非寄主抗性（NHR）是植物对其他物种病原微生物的最常见抗性形式。玉米小斑病菌是一种非适应性病原菌，会影响大豆，尤其是玉米/大豆间作系统中的大豆。然而，尚无实验证据描述大豆对玉米小斑病菌的免疫反应。为了阐明大豆非寄主抗性的分子机制，基于二维聚丙烯酰胺凝胶电泳（2-DE）进行了蛋白质组学分析，以鉴定参与大豆对玉米小斑病菌反应的蛋白质。玉米小斑病菌孢子在大豆叶片上的扩散诱导了整株植物的非寄主抗性，这促使几乎所有细胞器和各种代谢过程对玉米小斑病菌作出反应。发现一些酶，包括核酮糖-1,5-二磷酸羧化酶/加氧酶（RuBisCO）、线粒体加工肽酶（MPP）、放氧增强蛋白（OEE）和核苷二磷酸激酶（NDK），与大豆的非寄主抗性有关。这些酶在先前的研究中已被鉴定，STRING分析表明，大多数与主要代谢过程相关的蛋白质功能是作为对玉米小斑病菌的反应而被诱导的，这表明大豆和玉米小斑病菌之间存在一系列复杂的相互作用。这些发现表明大豆对非适应性病原菌存在系统性的非寄主抗性。这种反应的特征是代谢过程与对刺激的反应之间存在重叠。几个代谢过程为大豆提供了对非适应性病原菌玉米小斑病菌的先天免疫力。这项对大豆非寄主抗性的研究调查可能有助于更好地理解植物先天免疫，以及间作系统中植物与非适应性病原菌之间的相互作用。
