Lee Kevin C Y, Morgan Xochitl C, Power Jean F, Dunfield Peter F, Huttenhower Curtis, Stott Matthew B
GNS Science, Extremophiles Research Group, Taupō, New Zealand.
Department of Biostatistics, Harvard School of Public Health, Boston, MA USA ; Broad Institute of Harvard and MIT, Cambridge, MA USA.
Stand Genomic Sci. 2015 Nov 14;10:101. doi: 10.1186/s40793-015-0099-5. eCollection 2015.
Strain K22(T) is the type species of the recently- described genus Pyrinomonas, in subdivision 4 of the phylum Acidobacteria (Int J Syst Evol Micr. 2014; 64(1):220-7). It was isolated from geothermally-heated soil from Mt. Ngauruhoe, New Zealand, using low-nutrient medium. P. methylaliphatogenes K22(T) has a chemoheterotrophic metabolism; it can hydrolyze a limited range of simple carbohydrates and polypeptides. Its cell membrane is dominated by iso-branching fatty acids, and up to 40 % of its lipid content is membrane-spanning and ether lipids. It is obligately aerobic, thermophilic, moderately acidophilic, and non-spore-forming. The 3,788,560 bp genome of P. methylaliphatogenes K22(T) has a G + C content of 59.36 % and contains 3,189 protein-encoding and 55 non-coding RNA genes. Genomic analysis was consistent with nutritional requirements; in particular, the identified transporter classes reflect the oligotrophic nature of this strain.
菌株K22(T)是最近描述的吡啉单胞菌属的模式种，属于酸杆菌门第4亚群（《国际系统与进化微生物学杂志》。2014年；64(1):220 - 227）。它是从新西兰瑙鲁霍伊山的地热加热土壤中分离出来的，使用的是低营养培养基。甲基脂肪生成吡啉单胞菌K22(T)具有化能异养代谢；它能水解有限范围的简单碳水化合物和多肽。其细胞膜以异分支脂肪酸为主，其脂质含量的高达40%是跨膜醚脂。它是专性需氧、嗜热、中度嗜酸且不形成芽孢的。甲基脂肪生成吡啉单胞菌K22(T)的3,788,560 bp基因组的G + C含量为59.36%，包含3,189个蛋白质编码基因和55个非编码RNA基因。基因组分析与营养需求一致；特别是，鉴定出的转运蛋白类别反映了该菌株的贫营养性质。
