Shin Seoungwoo, Son Dahee, Kim Minkyung, Lee Seungjun, Roh Kyung-Baeg, Ryu Dehun, Lee Jongsung, Jung Eunsun, Park Deokhoon
Biospectrum Life Science Institute, Eines Platz 11th FL, 442-13 Sangdaewon Dong, Seoungnam City, Gyunggi Do 462-807, Korea.
Department of Genetic Engineering, Sungkyunkwan University, 2066, Seobu-Ro, Jangan Gu, Suwon City, Gyunggi Do 164-19, Korea.
Nutrients. 2015 Nov 12;7(11):9337-52. doi: 10.3390/nu7115478.
The accumulation of free radicals and advanced glycation end products (AGEs) in the skin plays a very important role in skin aging. Both are known to interact with each other. Therefore, natural compounds or extracts that possess both antioxidant and antiglycation activities might have great antiageing potential. Akebia quinata fruit extract (AQFE) has been used to treat urinary tract inflammatory disease in traditional Korean and Chinese medicines. In the present study, AQFE was demonstrated to possess antioxidant and antiglycation activity. AQFE protects human dermal fibroblasts (HDFs) from oxidative stress and inhibits cellular senescence induced by oxidative stress. We also found that AQFE inhibits glycation reaction between BSA and glucose. The antiglycation activity of AQFE was dose-dependent. In addition, the antiglycation activity of AQFE was confirmed in a human skin explant model. AQFE reduced CML expression and stimulated fibrillin-1 expression in comparison to the methyglyoxal treatment. In addition, the possibility of the extract as an anti-skin aging agent has also been clinically validated. Our analysis of the crow's feet wrinkle showed that there was a decrease in the depth of deep furrows in RI treated with AQFE cream over an eight-week period. The overall results suggest that AQFE may work as an anti-skin aging agent by preventing oxidative stress and other complications associated with AGEs formation.
自由基和晚期糖基化终产物（AGEs）在皮肤中的积累在皮肤衰老过程中起着非常重要的作用。已知两者会相互作用。因此，兼具抗氧化和抗糖基化活性的天然化合物或提取物可能具有巨大的抗老化潜力。木通果提取物（AQFE）在传统韩药和中药中已被用于治疗泌尿系统炎症性疾病。在本研究中，AQFE被证明具有抗氧化和抗糖基化活性。AQFE可保护人皮肤成纤维细胞（HDFs）免受氧化应激，并抑制由氧化应激诱导的细胞衰老。我们还发现AQFE可抑制牛血清白蛋白（BSA）与葡萄糖之间的糖基化反应。AQFE的抗糖基化活性呈剂量依赖性。此外，AQFE的抗糖基化活性在人皮肤外植体模型中得到了证实。与甲基乙二醛处理相比，AQFE降低了羧甲基赖氨酸（CML）的表达并刺激了原纤蛋白-1的表达。此外，该提取物作为抗皮肤衰老剂的可能性也已得到临床验证。我们对鱼尾纹的分析表明，在为期八周的时间里，用AQFE乳膏治疗的右侧（RI）深皱纹深度有所减少。总体结果表明，AQFE可能通过预防氧化应激和与AGEs形成相关的其他并发症而起到抗皮肤衰老剂的作用。
