Sornplang Pairat, Leelavatcharamas Vichai, Soikum Chaiyaporn
Fermentation research center for value added agricultural products, Khon Kaen University, Khon Kaen 40002, Thailand.
Asian-Australas J Anim Sci. 2015 Nov;28(11):1657-61. doi: 10.5713/ajas.15.0359.
Newborn chicks are susceptible to Salmonella enterica serovar Enteritidis (SE). The objective of this study was to evaluate the effect of Lactobacillus probiotic isolated from chicken feces on heterophil phagocytosis in broiler chicks. A total of 150 newborn broiler chicks were divided into 5 groups (30 chicks per group) as follows: group 1 (normal control), given feed and water only, group 2 (positive control) given feed, water and SE infection, group 3 (L61 treated) given feed, water, SE infection followed by Lactobacillus salivarius L61 treatment, group 4 (L55 treated) given feed, water, SE infection followed by L. salivarius L55 treatment, and group 5 given feed, water, SE infection followed by L. salivarius L61 + L55 combination treatment. After SE infection, L. salivarius treatment lasted for 7 days. The results showed that L. salivarius L61 and L. salivarius L55 treatment, either alone or combination of both, increased the survival rate after SE infection, and upregulated heterophil phagocytosis and phagocytic index (PI). Conversely, chick groups treated with Lactobacillus showed lower SE recovery rate from cecal tonsils than that of the positive control group. The PI values of the chicken group with SE infection, followed by the combination of L. salivarius L61 and L. salivarius L55 were the highest as compared to either positive control or normal control group. Two Lactobacillus strains supplementation group showed significantly (p<0.05) higher PI value at 48 h than 24 h after treatment.
新生雏鸡易感染肠炎沙门氏菌肠炎血清型（SE）。本研究的目的是评估从鸡粪便中分离出的乳酸杆菌益生菌对肉鸡雏鸡嗜异性粒细胞吞噬作用的影响。总共150只新生肉鸡雏鸡被分为5组（每组30只雏鸡），如下：第1组（正常对照组），仅给予饲料和水；第2组（阳性对照组），给予饲料、水并感染SE；第3组（L61处理组），给予饲料、水，感染SE后用唾液乳杆菌L61处理；第4组（L55处理组），给予饲料、水，感染SE后用唾液乳杆菌L55处理；第5组，给予饲料、水，感染SE后用唾液乳杆菌L61 + L55联合处理。SE感染后，唾液乳杆菌处理持续7天。结果表明，唾液乳杆菌L61和唾液乳杆菌L55单独或联合处理均提高了SE感染后的存活率，并上调了嗜异性粒细胞吞噬作用和吞噬指数（PI）。相反，用乳酸杆菌处理的雏鸡组盲肠扁桃体的SE回收率低于阳性对照组。与阳性对照组或正常对照组相比，感染SE后再用唾液乳杆菌L61和唾液乳杆菌L55联合处理的鸡组的PI值最高。两个乳酸杆菌菌株补充组在处理后48小时的PI值显著（p<0.05）高于24小时。
