Anudeep Sandanamudi, Prasanna Vaddi K, Adya Shruthi M, Radha Cheruppanpullil
Protein Chemistry and Technology Department, CSIR-Central Food Technological Research Institute, Mysuru 570 020, India.
Biochemistry and Nutrition Department, CSIR-Central Food Technological Research Institute, Mysuru 570 020, India.
Int J Biol Macromol. 2016 Oct;91:656-62. doi: 10.1016/j.ijbiomac.2016.06.013. Epub 2016 Jun 7.
Moringa oleifera (moringa or drumstick) seeds are a potential source of dietary fiber with 6.5% w/w soluble dietary fiber. Biochemical characterization of moringa seed soluble fiber revealed that it is a glycoprotein with 5% neutral sugars. Arabinose and xylose are the major neutral sugars identified by gas liquid chromatography (GLC). Moringa seed soluble fiber was identified as protease resistant-glycoprotein and termed as moringa seed resistant protein (MSRP). MSRP was found to be a homodimer (18kDa) containing two 9kDa monomeric units as revealed by SDS-PAGE analysis with pI 10.8. Immunostimulating activity of MSRP was assessed by murine splenocyte proliferation and production of NO from macrophages. MSRP at low concentration (0.01μg/well) strongly increased proliferation of splenocytes, while MSRP at high concentration weakly responded. MSRP induced 6-fold increase in NO production when compared to the control which indicates the activation of macrophages. MSRP isolated from defatted moringa seed flour is a potent mitogen, enhancing the proliferation of lymphocytes and inducing NO from macrophages. This study concludes that moringa seed is a potential nutritional source to promote the immune system of the host.
辣木籽是膳食纤维的潜在来源，其水溶性膳食纤维含量为6.5%（w/w）。对辣木籽水溶性纤维的生化特性分析表明，它是一种含有5%中性糖的糖蛋白。通过气液色谱法（GLC）鉴定出阿拉伯糖和木糖是主要的中性糖。辣木籽水溶性纤维被鉴定为抗蛋白酶糖蛋白，并被称为辣木籽抗性蛋白（MSRP）。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析显示，MSRP是一种同型二聚体（18kDa），包含两个9kDa的单体单元，其等电点为10.8。通过小鼠脾细胞增殖和巨噬细胞产生一氧化氮（NO）来评估MSRP的免疫刺激活性。低浓度（0.01μg/孔）的MSRP能强烈促进脾细胞增殖，而高浓度的MSRP反应较弱。与对照组相比，MSRP可使NO产生量增加6倍，这表明巨噬细胞被激活。从脱脂辣木籽粉中分离出的MSRP是一种有效的促有丝分裂原，可增强淋巴细胞的增殖并诱导巨噬细胞产生NO。本研究得出结论，辣木籽是促进宿主免疫系统的潜在营养来源。
