Zhou Bing-Rong, Zhang Ting, Bin Jameel Afzaal Ahmed, Xu Yang, Xu Yan, Guo Shi-Lei, Wang Ying, Permatasari Felicia, Luo Dan
a Department of Dermatology , the First Affiliated Hospital of Nanjing Medical University , Nanjing , China.
b Nanjing Regenerative Medicine Engineering and Technology Research Center , Nanjing , China.
J Cosmet Laser Ther. 2016 Jun;18(3):138-48. doi: 10.3109/14764172.2015.1114638. Epub 2016 Mar 8.
To evaluate the effects of conditioned medium of adipose-derived stem cells (ADSC-CM) on efficacy and side effects after fractional carbon dioxide laser resurfacing (FxCR) when treating subjects with facial atrophic acne scars or with skin rejuvenation needs.
Twenty-two subjects were enrolled in the study and divided into two groups. Nine subjects were included in skin rejuvenation group and thirteen subjects were included in acne scar group, and all subjects underwent three sessions of FxCR. ADSC-CM was applied on FxCR site of one randomly selected face side. Evaluations were done at baseline, 1 week after first treatment, and 1 month after each treatment. The outcome assessments included subjective satisfaction scale; blinded clinical assessment; and the biophysical parameters of roughness, elasticity, skin hydration, transepidermal water loss (TEWL), and the erythema and melanin index. Biopsies taken from one subject in skin rejuvenation group were analyzed using hematoxylin and eosin, Masson's Trichrome, and Gomori's aldehyde fuchsin staining.
ADSC-CM combined with FxCR increased subject satisfaction, elasticity, skin hydration, and skin elasticity and decreased TEWL, roughness, and the melanin index in both acne scars and skin rejuvenation groups. Histologic analysis showed that ADSC-CM increased dermal collagen density, elastin density, and arranged them in order.
ADSC-CM with FxCR is a good combination therapy for treating atrophic acne scars and skin rejuvenation.
JSPH2012-082 - Registered 14 Feb 2012.
评估脂肪来源干细胞条件培养基（ADSC-CM）在分次二氧化碳激光换肤术（FxCR）治疗面部萎缩性痤疮瘢痕或有皮肤年轻化需求的受试者时，对疗效和副作用的影响。
22名受试者纳入本研究并分为两组。皮肤年轻化组9名受试者，痤疮瘢痕组13名受试者，所有受试者均接受3次FxCR治疗。将ADSC-CM应用于随机选择的一侧面部的FxCR治疗部位。在基线、首次治疗后1周以及每次治疗后1个月进行评估。结局评估包括主观满意度量表；盲法临床评估；以及粗糙度、弹性、皮肤水合作用、经表皮水分流失（TEWL）、红斑和黑色素指数等生物物理参数。对皮肤年轻化组的1名受试者进行活检，采用苏木精-伊红染色、Masson三色染色和Gomori醛复红染色进行分析。
ADSC-CM联合FxCR提高了痤疮瘢痕组和皮肤年轻化组受试者的满意度、弹性、皮肤水合作用和皮肤弹性，降低了TEWL、粗糙度和黑色素指数。组织学分析表明，ADSC-CM增加了真皮胶原密度、弹性蛋白密度，并使其排列有序。
ADSC-CM联合FxCR是治疗萎缩性痤疮瘢痕和皮肤年轻化的良好联合治疗方法。
JSPH2012-082 - 于2012年2月14日注册。
