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纳米孔测序作为一种可快速部署的埃博拉疫情应对工具。

Nanopore Sequencing as a Rapidly Deployable Ebola Outbreak Tool.

作者信息

Hoenen Thomas, Groseth Allison, Rosenke Kyle, Fischer Robert J, Hoenen Andreas, Judson Seth D, Martellaro Cynthia, Falzarano Darryl, Marzi Andrea, Squires R Burke, Wollenberg Kurt R, de Wit Emmie, Prescott Joseph, Safronetz David, van Doremalen Neeltje, Bushmaker Trenton, Feldmann Friederike, McNally Kristin, Bolay Fatorma K, Fields Barry, Sealy Tara, Rayfield Mark, Nichol Stuart T, Zoon Kathryn C, Massaquoi Moses, Munster Vincent J, Feldmann Heinz

出版信息

Emerg Infect Dis. 2016 Feb;22(2):331-4. doi: 10.3201/eid2202.151796.

DOI:10.3201/eid2202.151796
PMID:26812583
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4734547/
Abstract

Rapid sequencing of RNA/DNA from pathogen samples obtained during disease outbreaks provides critical scientific and public health information. However, challenges exist for exporting samples to laboratories or establishing conventional sequencers in remote outbreak regions. We successfully used a novel, pocket-sized nanopore sequencer at a field diagnostic laboratory in Liberia during the current Ebola virus outbreak.

摘要

在疾病暴发期间,对从病原体样本中获取的RNA/DNA进行快速测序可提供关键的科学和公共卫生信息。然而,将样本运往实验室或在偏远的疫情地区建立传统测序仪存在挑战。在当前埃博拉病毒暴发期间,我们在利比里亚的一个现场诊断实验室成功使用了一种新型的袖珍纳米孔测序仪。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f5/4734547/f276f6b1159a/15-1796-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f5/4734547/655924eaeed4/15-1796-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f5/4734547/f276f6b1159a/15-1796-F2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f5/4734547/655924eaeed4/15-1796-F1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f5/4734547/f276f6b1159a/15-1796-F2.jpg

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Genome Med. 2015 Sep 29;7:99. doi: 10.1186/s13073-015-0220-9.
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Delayed Disease Progression in Cynomolgus Macaques Infected with Ebola Virus Makona Strain.感染埃博拉病毒马科纳毒株的食蟹猴疾病进展延迟
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