在微量滴定系统中比较水牛绿猴细胞和 McCoy 细胞用于沙眼衣原体分离的情况。
Comparison of buffalo green monkey cells and McCoy cells for isolation of Chlamydia trachomatis in a microtiter system.
作者信息
Krech T, Bleckmann M, Paatz R
机构信息
Institute for Medical Microbiology and Virology, University of Düsseldorf, Federal Republic of Germany.
出版信息
J Clin Microbiol. 1989 Oct;27(10):2364-5. doi: 10.1128/jcm.27.10.2364-2365.1989.
Abstract
A total of 1,229 urogenital samples of patients with sexually transmitted diseases and prostitutes were inoculated simultaneously in McCoy cells and Buffalo green monkey (BGM) cells by using a microtitration technique. BGM cells proved to be slightly more sensitive than McCoy cells, and they usually showed more and larger inclusions and were more resistant to cytotoxic substances in samples. The decrease in sensitivity as a result of mycoplasmal contamination was much more prominent in McCoy cells than in BGM cells.
摘要
采用微量滴定技术，将1229份性传播疾病患者和妓女的泌尿生殖系统样本同时接种于 McCoy 细胞和水牛绿猴（BGM）细胞中。结果表明，BGM 细胞比 McCoy 细胞稍敏感，通常表现出更多、更大的包涵体，且对样本中的细胞毒性物质更具抗性。支原体污染导致的敏感性降低在 McCoy 细胞中比在 BGM 细胞中更为显著。
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Comparison of buffalo green monkey cells and McCoy cells for isolation of Chlamydia trachomatis in a microtiter system.在微量滴定系统中比较水牛绿猴细胞和 McCoy 细胞用于沙眼衣原体分离的情况。
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