Pang Bo, Du Pengcheng, Zhou Zhemin, Diao Baowei, Cui Zhigang, Zhou Haijian, Kan Biao
National Institute for Communicable Disease Control and Prevention, China CDC, Beijing, 102206, People's Republic of China.
State Key Laboratory of Infectious Disease Prevention and Control, Beijing, 102206, People's Republic of China.
PLoS One. 2016 Mar 1;11(3):e0149742. doi: 10.1371/journal.pone.0149742. eCollection 2016.
Vibrio cholerae has caused massive outbreaks and even trans-continental epidemics. In 2008 and 2010, at least 3 remarkable cholera outbreaks occurred in Hainan, Anhui and Jiangsu provinces of China. To address the possible transmissions and the relationships to the 7th pandemic strains of those 3 outbreaks, we sequenced the whole genomes of the outbreak isolates and compared with the global isolates from the 7th pandemic. The three outbreaks in this study were caused by a cluster of V. cholerae in clade 3.B which is parallel to the clade 3.C that was transmitted from Nepal to Haiti and caused an outbreak in 2010. Pan-genome analysis provided additional evolution information on the mobile element and acquired multiple antibiotic resistance genes. We suggested that clade 3.B should be monitored because the multiple antibiotic resistant characteristics of this clade and the 'amplifier' function of China in the global transmission of current Cholera pandemic. We also show that dedicated whole genome sequencing analysis provided more information than the previous techniques and should be applied in the disease surveillance networks.
霍乱弧菌已引发大规模疫情，甚至跨洲际流行。2008年和2010年，中国海南、安徽和江苏三省至少发生了3起显著的霍乱疫情。为了探讨这3起疫情的可能传播途径以及与第七次霍乱大流行菌株的关系，我们对疫情分离株进行了全基因组测序，并与来自第七次霍乱大流行的全球分离株进行了比较。本研究中的三起疫情是由进化枝3.B中的一群霍乱弧菌引起的，该进化枝与从尼泊尔传播到海地并于2010年引发疫情的进化枝3.C平行。泛基因组分析提供了关于移动元件的更多进化信息，并获得了多个抗生素抗性基因。我们建议应对进化枝3.B进行监测，因为该进化枝具有多重抗生素抗性特征，以及中国在当前霍乱大流行全球传播中的“放大器”作用。我们还表明，专门的全基因组测序分析比以前的技术提供了更多信息，应应用于疾病监测网络。
