Kohman Richie E, Cha Susie S, Man Heng-Ye, Han Xue
Biomedical Engineering Department, Boston University , Boston, Massachusetts 02215, United States.
Biology Department, Boston University , Boston, Massachusetts 02215, United States.
Nano Lett. 2016 Apr 13;16(4):2781-5. doi: 10.1021/acs.nanolett.6b00530. Epub 2016 Mar 3.
Recent innovations in DNA nanofabrication allow the creation of intricately shaped nanostructures ideally suited for many biological applications. To advance the use of DNA nanotechnology for the controlled release of bioactive molecules, we report a general strategy that uses light to liberate encapsulated cargoes from DNA nanostructures with high spatiotemporal precision. Through the incorporation of a custom, photolabile cross-linker, we encapsulated cargoes ranging in size from small molecules to full-sized proteins within DNA nanocages and then released such cargoes upon brief exposure to light. This novel molecular uncaging technique offers a general approach for precisely releasing a large variety of bioactive molecules, allowing investigation into their mechanism of action, or finely tuned delivery with high temporal precision for broad biomedical and materials applications.
DNA纳米制造领域的最新创新使得能够创建形状复杂的纳米结构，这些结构非常适合许多生物应用。为了推动DNA纳米技术在生物活性分子控释方面的应用，我们报告了一种通用策略，该策略利用光以高时空精度从DNA纳米结构中释放封装的货物。通过引入一种定制的光不稳定交联剂，我们将从小分子到全尺寸蛋白质等不同大小的货物封装在DNA纳米笼中，然后在短暂光照后释放这些货物。这种新型的分子解笼技术提供了一种精确释放多种生物活性分子的通用方法，可用于研究其作用机制，或为广泛的生物医学和材料应用提供具有高时间精度的精细调控递送。
