Zhu Weidong, Lee Seok-Woo
Formerly, Department of Medicine, University of California School of Medicine, Los Angeles, CA, USA .
Departments of Dental Education and Periodontology, Dental Science Research Institute, Chonnam National University School of Dentistry, Gwangju, Korea .
J Periodontal Implant Sci. 2016 Feb;46(1):2-9. doi: 10.5051/jpis.2016.46.1.2. Epub 2016 Feb 15.
Porphyromonas gingivalis and Tannerella forsythia have been implicated as the major etiologic agents of periodontal disease. These two bacteria are frequently isolated together from the periodontal lesion, and it has been suggested that their interaction may increase each one's virulence potential. The purpose of this study was to identify proteins on the surface of these organisms that are involved in interbacterial binding.
Biotin labeling of surface proteins of P. gingivalis and T. forsythia and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed to identify surface proteins involved in the coaggregating activity between P. gingivalis and T. forsythia.
It was found that three major T. forsythia proteins sized 161, 100, and 62 kDa were involved in binding to P. gingivalis, and P. gingivalis proteins sized 35, 32, and 26 kDa were involved in binding to T. forsythia cells.
LC-MS/MS analysis identified one T. forsythia surface protein (TonB-linked outer membrane protein) involved in interbacterial binding to P. gingivalis. However, the nature of other T. forsythia and P. gingivalis surface proteins identified by biotin labeling could not be determined. Further analysis of these proteins will help elucidate the molecular mechanisms that mediate coaggregation between P. gingivalis and T. forsythia.
牙龈卟啉单胞菌和福赛坦氏菌被认为是牙周病的主要病原体。这两种细菌经常从牙周病变部位共同分离出来，有人提出它们之间的相互作用可能会增加彼此的毒力潜能。本研究的目的是鉴定这些生物体表面参与细菌间结合的蛋白质。
对牙龈卟啉单胞菌和福赛坦氏菌的表面蛋白进行生物素标记，并进行液相色谱-串联质谱（LC-MS/MS）分析，以鉴定参与牙龈卟啉单胞菌和福赛坦氏菌之间共聚活性的表面蛋白。
发现三种分子量分别为161 kDa、100 kDa和62 kDa的主要福赛坦氏菌蛋白参与与牙龈卟啉单胞菌的结合，而分子量为35 kDa、32 kDa和26 kDa的牙龈卟啉单胞菌蛋白参与与福赛坦氏菌细胞的结合。
LC-MS/MS分析鉴定出一种参与与牙龈卟啉单胞菌细菌间结合的福赛坦氏菌表面蛋白（TonB连接的外膜蛋白）。然而，通过生物素标记鉴定出的其他福赛坦氏菌和牙龈卟啉单胞菌表面蛋白的性质尚无法确定。对这些蛋白的进一步分析将有助于阐明介导牙龈卟啉单胞菌和福赛坦氏菌之间共聚的分子机制。
