R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University , Chung-Li, Taoyuan 320, Taiwan, R.O.C.
Department of Botany and Microbiology, College of Science, King Saud University , P.O. Box 2455, Riyadh 11451, Saudi Arabia.
Langmuir. 2017 Sep 26;33(38):9822-9835. doi: 10.1021/acs.langmuir.7b02164. Epub 2017 Sep 5.
Titanium and stainless steel materials are widely used in numerous devices or in custom parts for their excellent mechanical properties. However, their lack of biocompatibility seriously limits their usage in the biomedical field. This study focuses on the grafting of triblock copolymers on titanium and stainless steel metal susbtrates for improving their general biofouling resistance. The series of copolymers that we designed is composed of two blocks of zwitterionic sulfobetaine (SBMA) monomers and one block of glycidyl methacrylate (GMA). The number of repeat units forming each block, n, was finely tuned and controlled to 25, 50, 75, or 100, permitting regulation of the grafting thickness, the morphology, and the dependent properties such as the surface hydrophilicity and biofouling resistance. It was shown that the copolymer possessing n = 50 repeat units in each block, corresponding to a molecular weight of about 15.2 kDa, led to the best nonfouling properties, assessed using plasma proteins, blood cells, fibroblasts cells, and various bacteria. This was explained by an optimized grafting degree and chain organization of the copolymer. Lower value (n = 25) and higher values (n = 75, 100) led to low surface coverage and the formation of aggregates, respectively. The best copolymer was grafted onto scalpels (steel) and dental roots (titanium), and antifouling properties demonstrated using Escherichia coli and HT1080 cells. Results of this work show that this unique triblock copolymer holds promise as a potential material for surface modification of biomedical metallic devices, provided a fine-tuning of the blocks organization and length.
钛和不锈钢材料因其优异的机械性能而被广泛应用于许多设备或定制部件中。然而，它们缺乏生物相容性严重限制了它们在生物医学领域的应用。本研究专注于在钛和不锈钢金属基底上接枝嵌段共聚物以提高其一般抗生物污垢性能。我们设计的共聚物系列由两个聚（甜菜碱甲基丙烯酰胺）（SBMA）单体嵌段和一个甲基丙烯酸缩水甘油酯（GMA）嵌段组成。形成每个嵌段的重复单元数 n 被精细地调谐和控制在 25、50、75 或 100，从而可以调节接枝厚度、形态以及依赖于表面亲水性和抗生物污垢性能等的相关性能。结果表明，具有每个嵌段 50 个重复单元的共聚物（对应于约 15.2 kDa 的分子量）导致了最佳的非污垢性能，使用血浆蛋白、血细胞、成纤维细胞和各种细菌进行了评估。这可以通过共聚物的优化接枝度和链组织来解释。较低的值（n = 25）和较高的值（n = 75、100）分别导致低表面覆盖率和聚集的形成。最佳共聚物接枝到手术刀（钢）和牙根管（钛）上，并使用大肠杆菌和 HT1080 细胞证明了抗污垢性能。这项工作的结果表明，这种独特的嵌段共聚物作为生物医学金属设备表面改性的潜在材料具有很大的应用前景，前提是对嵌段的组织和长度进行精细的调整。
