Barrett T, Belsham G J, Subbarao S M, Evans S A
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, United Kingdom.
Virology. 1989 May;170(1):11-8. doi: 10.1016/0042-6822(89)90346-2.
A cDNA clone containing the complete coding sequence of the rinderpest fusion protein (F) gene was inserted into the thymidine kinase gene of vaccinia virus (WR strain) under the control of the 7.5K early/late vaccinia virus promoter. All forms of the F protein, i.e., the glycosylated F0 precursor, the unglycosylated F1 protein, and the glycosylated F2 protein, were detected in cells infected with the recombinant virus. Vaccination of rabbits with the recombinant virus induced antibodies which reacted in an ELISA system specific for rinderpest. The rabbit sera contained neutralizing antibodies against rinderpest virus and precipitated the F protein from lysates of rinderpest infected cells. Rabbits vaccinated with the recombinant rinderpest F gene vaccinia virus were protected from a lethal challenge with the lapinized Nakamura 3 strain of rinderpest virus. Variations in the severity of clinical symptoms correlated with the level of anti-F protein antibodies produced.
将含有牛瘟融合蛋白（F）基因完整编码序列的cDNA克隆，在痘苗病毒7.5K早期/晚期启动子的控制下，插入痘苗病毒（WR株）的胸苷激酶基因中。在感染重组病毒的细胞中检测到了F蛋白的所有形式，即糖基化的F0前体、未糖基化的F1蛋白和糖基化的F2蛋白。用重组病毒对兔子进行免疫接种可诱导产生在牛瘟特异性ELISA系统中发生反应的抗体。兔血清中含有抗牛瘟病毒的中和抗体，并能从牛瘟感染细胞的裂解物中沉淀出F蛋白。用重组牛瘟F基因痘苗病毒免疫接种的兔子，可免受兔化中村3型牛瘟病毒的致死性攻击。临床症状严重程度的差异与所产生的抗F蛋白抗体水平相关。
